| Other Abstract | Objective:To investigate the effect of baicalin on apoptosis and the expression of apoptotic regulatory protein survivin in myocytes of type 2 experimental diabetic rats. So to ascertain the protection of baicalin to myocytes in diabetic rats.
Methods:Thirty healthy male Wistar rats were randomly divided into three groups: the normal control group(n=10),the diabetes group(n=10)and the baicalin group(n=10). The normal control group was given common diet while the other two groups were given high-fat,high-suger diet for six weeks, the diabetes group and the baicalin group received intraperitoneal injection of streptozotocin once in a dose of 30mg/kg.After one week,the diabetes mellitus rat model was established. At the same time,150 mg•kg-1•d-1 of baicalin was administrated by gavage to the baicalin groups,and the same volume normal saline was given to the normal control and the diabetes group for 8 weeks.All rats were weighted,then killed,and the fasting blood glucose,fasting insulin,triglyceride,cholesterol, high-density lipoprotein,low-density lipoprotein were determined. TdT-mediated dUTP nick end labeling was used for the determination of myocardial cell apoptosis.Reverse transcription-polymerase chain reaction was used for the determination of mRNA for survivin of myocardium. Immunohistochemistry was used for the determination of protein expression for survivin of myocardium.
Results:(1)Weight and serum indices:compared with the normal control group, fasting blood glucose,fasting insulin,triglyceride,cholesterol,low-density lipoprotein were significantly higher in the diabetes group and the baicalin group(P<0.05 or P<0.01),body weight, high-density lipoprotein were lower in the diabetes group and the baicalin group (P<0.01).Compared with the diabetes group, triglyceride,cholester-
ol,low-density lipoprotein were significantly lower(P<0.05) in the baicalin group, high-density lipoprotein was higher in the baicalin group(P<0.05), and body weight,fasting insulin,fasting blood glucose in the baicalin group was insignificantly different from the diabetes group(P＞0.05). (2) myocardial cell apoptosis:By TUNEL, compared with the normal control group, apoptosis index was significantly higher in the diabetes group and the baicalin group(P<0.01),compared with the diabetes group, apoptosis index was significantly lower(P<0.01) in the baicalin group.(3)The mRNA expression of survivin of myocardium:By RT-PCR, compared with the normal control group,t... |
