| 黄芩苷对2型糖尿病大鼠肾脏组织survivin表达以及细胞凋亡的影响 |
| Alternative Title | The effect of baicalin on expression of survivin and apoptosis in kidney of type 2 diabetic rats
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| 孙洁 |
| Thesis Advisor | 田林红
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| 2011-05-12
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 黄芩苷
2型糖尿病
糖尿病肾病
细胞凋亡
survivin
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| Abstract | 目的： 观察黄芩苷对2型糖尿病 (T2DM)大鼠肾组织凋亡及对凋亡调控基因survivin表达的影响，并探讨黄芩苷对糖尿病大鼠肾脏的作用及其作用机制。方法：健康成年Wistar雄性大鼠30只，采用随机数字表随机分为正常对照组（NC组,n=10）、糖尿病模型组（DM组,n=10）和黄芩苷干预组（BAI组,n=10）。NC组采用常规饲料喂养，余两组均予以高糖高脂饲料喂养。连续喂养6周后，高热量饲料喂养的大鼠禁食12小时，于左下腹腔注射2%的STZ溶液30mg/kg建立糖尿病实验动物模型。模型成功建立后，BAI组大鼠使用黄芩苷150mg/kg/ d 灌胃，而NC组和DM组予以生理盐水等体积灌胃，灌胃8周后所有大鼠称体重、肾重，并计算肾指数；8周末代谢笼留取24h尿液，做24小时尿蛋白定量。一侧肾脏被用于免疫组化检测及其凋亡率的测定，另一侧肾脏液氮保存后采用荧光实时定量逆转录聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR）方法检测survivin mRNA表达水平。结果：与NC组比较，DM组及BAI组空腹血糖、甘油三酯（TG）、胆固醇（CHO）、低密度脂蛋白（LDL-C）、肾质量、肾脏指数、24小时尿量以及尿蛋白定量及其凋亡指数明显升高（P<0.01），而体重、高密度脂蛋白（HDL-C）、survivin mRNA相对表达量和survivin 蛋白质水平均降低（P<0.01）。与DM组比较，BAI组甘油三酯（TG）、胆固醇（CHO）、低密度脂蛋白（LDL-C）、24小时尿量、尿蛋白定量及凋亡指数均减低（P<0.01）。 |
| Other Abstract | Objective: To investigate the effect of baicalin on apoptosis and the expression of apoptotic regulatory protein survivin in kidney of type 2 experimental diabetic rats. So to ascertain the anti -apoptosis of baicalin in the diabetic rat kidney.Methods: Thirty healthy male Wistar rats were divided randomly into three groups: the normal control group (n=10),the diabetes group(n=10)and the baicalin group(n=10). The normal rats were fed normal rat chow. while the other two groups were given high-fat, high-sugar diet for six weeks, the diabetes group and the baicalin group received intraperitoneal injection of streptozotocin once in a dose of 30mg/kg.After one week, the diabetes mellitus rat model was established. At the same time, 150 mg•kg-1•d-1 of baicalin was administrated to the baicalin groups by oral gavaging, and the same volume normal saline was given to the normal control and the diabetes group for 8 weeks. All rats were weighted, then killed, and the fasting blood glucose , triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein were determined. TdT-mediated dUTP nick end labeling was used for the determination of kidney cell apoptosis. Reverse transcription-polymerase chain reaction was used for the determination of mRNA for survivin of kidney. Immunohistochemistry was used for the determination of protein expression for survivin of kidney。Results: compared with the normal control group, fasting blood glucose, triglyceride, cholesterol, low-density lipoprotein, Absolute and relative weight of kidney, Urine Volume for 24h and Total protein ,poptosis index were all significantly higher in the diabetes group and the baicalin group (P<0.01), body weight, high-density lipoprotein ,the mRNA and protein expression of survivin of kidney was significantly lower in the diabetes group and the baicalin group (P<0.01). |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223296
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
孙洁. 黄芩苷对2型糖尿病大鼠肾脏组织survivin表达以及细胞凋亡的影响[D]. 兰州. 兰州大学,2011.
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