| 黄芪及其单体黄芪甲苷对代谢综合征和高血压心脏损害的影响 |
| Alternative Title | The Effect of Radix Astragali and Its Monomer Astragaloside IV on Cardiac Damage in Metabolic Syndrome and Hypertension
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| 王琼英 |
| Thesis Advisor | 余静
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| 2015-06-09
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 博士
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| Keyword | 黄芪
代谢综合征
氧化应激
Mas受体
左室舒张功能不全
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| Abstract | 黄芪对代谢综合征大鼠心脏血管紧张素1-7受体Mas表达的影响
目的：探讨黄芪影响代谢综合征（MS）大鼠心脏血管紧张素1-7（Ang 1-7）受体Mas蛋白的表达及其抗氧化应激的心肌保护作用II)、丙二醛（MDA）、超氧化物歧化酶（SOD）水平，免疫蛋白印迹法检测各组大鼠心肌Mas受体、血管紧张素转化酶（ACE）和ACE2的表达。结论：黄芪可以提高心肌组织 ACE2、Mas的表达, 降低ACE表达,改善心脏局部ACE、Mas的水平；采用2K1C结合高脂饮食，并配合果糖水诱导可建造以高血压为主的MS大鼠模型。
黄芪甲苷经eNOS/NO/cGMP通路改善代谢综合征大鼠左室舒张功能不全
目的:我们将探讨黄芪甲苷（AST）对高糖高脂饮食诱导的MS大鼠心脏保护作用及其机制。黄芪甲苷通过增加磷酸化内皮型一氧化氮合酶改善左室舒张功能不全
目的：对于舒张功能不全现在还没有特定有效的治疗，主要原因是因为人们对这类疾病发病机制的了解相对缺乏。AST 可以提高eNOS活性、清除活性氧自由基和NO的生成。我们假设AST可以改善因eNOS 的磷酸化水平降低导致的DOCA-盐敏性高血压小鼠左室舒张功能不全。结果：黄芪甲苷可以逆转DOCA-盐敏性高血压小鼠舒张功能不全.黄芪甲苷对高血压性心脏病大鼠室性心律失常及心功能作用机制。 |
| Other Abstract | Effect of Radix Astragali on the Expressions of Angiotensin 1-7 Receptorin Myocardium of Metabolic Syndrome Rats
Objective: To clarify the anti-oxidative role of Radix Astragali and its effects on the angiotensin (Ang) 1-7 specific receptor, Mas, in metabolic syndrome (MS) rats.Methods: A total of 160 male SD rats were divided into three groups: nomal control group (NC), MS group，MS+Radix Astragali group (MS+RA, 6 mg/kg/day in gavage) and MS+high dose Radix Astragali group (MS+HRA, 10 mg/kg/day in gavage). The two-kidney, one-clip method with high-fat diet and fructose water was constructed to set up the MS model. After four weeks treatment, the hemodynamic and echocardiographic parameters were used to assess the left ventricular functions. Plasma and myocardial AngⅡ, malondialdehyde and superoxide demutase (SOD) levels were measured with radioimmunoassay. Results: Compared with NC group, the systolic and diastolic pressure, body weight, fasting glucose, fasting insulin, triglycerides, free fatty acid, AngⅡ and myocardial malondialdehyde level(P<0.05) of MS , MS+RA and MS+HRA were significantly increased (P<0.05), while myocardial SOD activity was decreased (P<0.05).The plasma AngⅡ and myocardial malondialdehyde level of MS+RA and MS+HRA groups were lower than MS group (P=0.001, 0.017), while myocardial SOD activity was increased.Hemodynamic parameters showed that left ventricular internal pressure -dp/dt of MS+RA group wsa higher than MS group（P=0.037, 0.033）.ACE protein expression in myocardial tissue of MS group was increased than NC group,while ACE2 and Mas receptor expression were decreased（all P<0.05）.Compared with MS group,ACE protein expression in myocardial tissue was decreased,while ACE2 and Mas receptor expression were increased in MS+RA and MS+HRA groups（all P<0.05）.Conclusions: Astragali can increase the ACE2 and Mas receptor expressions through anti-oxidative effects. This has implications for new therapeutic in metabolic syndrome.
ABSTRACT PART 2 Astragaloside IV Improved Left Ventricular Diastolic Dysfunction in Metabolic Syndrome Rats through eNOS/NO/cGMP Pathway
Objective:Metabolic syndrome (MS) is a major risk factor for heart failure, initially manifesting as diastolic dysfunction. In this study, the effects and mechanisms of astragaloside IV (AST) were investigated on high fructose/high fat diet (HFFD)-induced MS rats. Methods: The rat MS model was established by feeding HFFD for 24 weeks. Rats were assigned to four gro... |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223307
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
王琼英. 黄芪及其单体黄芪甲苷对代谢综合征和高血压心脏损害的影响[D]. 兰州. 兰州大学,2015.
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