| 黄芪多糖对白血病细胞MICA表达及NK细胞杀伤敏感性的影响 |
| Alternative Title | The effects of Astragalus polysaccharide on the expression of MICA in leukemia cell lines and its impact on NK cytotoxicity
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| 邓黎黎 |
| Thesis Advisor | 张连生
|
| 2012-05-23
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 黄芪多糖
白血病细胞
NKG2D
MICA
NK细胞
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| Abstract | 利用CCK-8法检测黄芪多糖对HL-60、K562细胞的半数抑制量(IC50)，以此含量作用HL-60、K562细胞48小时后，采用RT-PCR、流式细胞仪分别检测作用前后白血病细胞MICA基因和蛋白水平的表达变化。运用乳酸脱氢酶释放法检测NK细胞对黄芪多糖处理前后HL-60、K562细胞的杀伤率。
结果：经黄芪多糖处理后HL-60细胞MICA mRNA表达是处理前的1.46±0.16倍，在不同效靶比（5:1、10:1、20:1）下均有显著差异，差异有统计学意义( P<0.05) 。
结论：1.黄芪多糖作用后白血病细胞HL-60、K562的MICA mRNA及MICA表达均显著上调；2.黄芪多糖能可以通过上调白血病细胞MICA的表达增强白血病细胞对NK细胞的杀伤敏感性。 |
| Other Abstract | Objective: This study based on the observation of that if astragalus polysaccharides could increase the expression of MICA in HL-60, K562 cells and enhance the cytotoxicity of NK cell against leukemia cell lines, and to find new targets for NK cell-based immunotherapy in leukemia.
Methods: The different concentrations of astragalus polysaccharides incubated with the HL-60, effector-to-target cell ratios (E:T) before and after treating with APS.
Results: The MICA mRNA of HL-60 cells after treating with APS is 1.46±0.16 times than that of before treating with APS; MICA mRNA of K562 cells after treating with APS is 2.26±0.34 times than that of before ( P < 0.05). the difference have statistical significance ( P< 0.05).
Conclusion: The APS could enhance the sensitivity of leukemia cells to NK cell through the up-expression of MICA on the surface of leukemia cell. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223312
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
邓黎黎. 黄芪多糖对白血病细胞MICA表达及NK细胞杀伤敏感性的影响[D]. 兰州. 兰州大学,2012.
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