兰州大学机构库 >学院待认领
谷氨酰胺对急进高原缺氧大鼠肠道微生态影响的实验研究
Alternative TitleEffect of glutamine on change of intestinal microecology in rats exposedto acute plateau hypoxia
杨文翠
Thesis Advisor张方信
2010-05-20
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword谷氨酰胺 高原缺氧 肠道微生态 细胞因子
Abstract目的:探讨谷氨酰胺对急进不同高原环境大鼠肠道微生态(菌群失调和细菌易位)和细胞因子的影响,从而为急进不同高原环境防治肠道微生态失衡提供理论依据。方法:Wistar大鼠130只,按不同海拔随机分为五组:A组(对照组:海拔高度500 m,陕西,西安)、B组(海拔高度3848 m,榆中,马衔山)、C组(海拔高度3848 m,榆中,马衔山,谷氨酰胺干预)、D组(海拔高度4767m,可可西里,高原实验中心)和E组(海拔高度4767m,可可西里,高原实验中心,谷氨酰胺干预)。 A组10只大鼠;B组和C组每组各30只大鼠,急进3848 m高原,分别于第2、3、4d取材,每次取10只;D组和E组每组各30只大鼠,急进4767 m高原,分别于第2、3、4d取材,每次取10只。每组大鼠行粪便涂片油镜观察、粪便pH值测定、肠道细菌培养、细菌易位培养和肠黏膜组织细胞因子的测定。结果1 粪便涂片检测肠道菌群A组大鼠肠道菌群失衡率为10%,B、D组大鼠肠道菌群失衡率分别是53%和86%,与A组比较差异均有统计学意义(P<0.05)。谷氨酰胺干预后,C、E组大鼠肠道菌群失衡率分别是46%和70%,与相应高原组比较,差异有统计学意义(P<0.05)。2 A组粪便pH为20%,B、D组粪便pH分别为53%和80%,与A组比较差异均有统计学意义(P<0.05)。谷氨酰胺干预后,C、E组大鼠粪便pH分别是40%和67%,与相应高原组比较,差异有统计学意义(P<0.05)。3 B组细菌易位率与A组比较无统计学差异(P>0.05),D组细菌易位率与A组比较增高,有统计学差异(P<0.05)。谷氨酰胺干预后,C、D组细菌易位率与相应高原比较有统计学差异(P<0.05)。4 B、D组肠组织TNF-α和IL-6的含量均高于A组(P<0.05)。与B组比较,C组TNF-α和IL-6的含量减少,差异有统计学意义(P<0.05)。与D组比较,E组TNF-α和IL-6的含量减少,差异有统计学意义(P<0.05)。结论:急进高原缺氧环境可导致大鼠肠道菌群失衡及细菌易位的发生,细胞因子表达增高,肠黏膜组织损伤,肠黏膜屏障破坏,且随着海拔增高和时间延长上述改变更明显。谷氨酰胺具有保护肠黏膜屏障及调节肠道菌群失衡的作用。
Other AbstractObjective To observe cytokine and microecology (dysbacteriosis and bacterial translocation) changes of intestinal mucosa of glutamine (GLN) in rats exposed to acute high altitude. It can provide theoretical basis to prevent the imbalance of intestinal microflora in radical plateau hypoxia environment. Methods Wistar rats of 130 were randomly divided into A group(control group, altitude500 meters, Shanxi, xian), B group (altitude 3848 meters, Yuzhong, Maxian mountain), C group (altitude 3848 meters of glutamine intervention, Yuzhong, Maxian mountain), D group (altitude 4767 meters, plateau experiment center in Kekexili) and E group (altitude 4767 meters, plateau experiment center of glutamine intervention in Kekexili). A group have 10 rats. B and C groups were posed acute plateau hypoxia model to altitude 3848 meters, We drawn on the 2th, 3th and 4th day, which kill 10 rats. D and E groups were posed acute plateau hypoxia model to altitude 4767 meters, We drawn on the 2th, 3th and 4th day, which kill 10 rats. To detect the changes of feces smears oil microscopic observation, faeces pH value, dysbacteriosis and bacterial translocation and cytokine. Results 1 Intestinal flora imbalance rate in A group was 10%. B and D groups were discernly 53% and 86%, which were statistically significant than A group(P<0.05). C and E groups of glutamine intervention were discernly 46% and 70%, which were statistically significant than corresp plateau group(P<0.05). 2 Faeces pH value in A group was 10%. B and D groups were discernly 53% and 80%, which were statistically significant than A group(P<0.05). C and E groups of glutamine intervention were discernly 40% and 67%, which were statistically significant than corresp plateau group(P<0.05). 3 Intestinal bacterial translocation rate in B group was not significant than A group(P>0.05). Intestinal bacterial translocation rate in D group was significant than A group(P<0.05). Intestinal bacterial translocation rates in C and E groups of glutamine intervention were significant than corresp plateau group(P<0.05). 4 The TNF-α and IL-6 contents of intestinal tissue in B and D groups were significantly higher than A group. The TNF-α and IL-6 contents of intestinal tissue in C group was lower than B group, the difference has statistincal significance(P<0.05). The TNF-α and IL-6 contents of intestinal tissue in E group was lower than D group, the difference has statistincal significance(P<0.05). Conclusion Exposure...
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223395
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
杨文翠. 谷氨酰胺对急进高原缺氧大鼠肠道微生态影响的实验研究[D]. 兰州. 兰州大学,2010.
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