兰州大学机构库 >学院待认领
高原习服中大鼠肝脏葡萄糖、脂肪酸代谢特点及机制
Alternative TitleCharacteristics and mechanisms of hepatic glucose and fatty acid metabolism in rats during high altitude acclimatization
倪倩
Thesis Advisor张有成
2014-12-05
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name博士
Keyword高原 三羧酸循环 糖异生 脂肪酸氧化 脂肪酸合成 肝脏
Abstract目的 探讨高原习服中(海拔4300m,1天、3天、7天、15天、30天)非运动状态下大鼠肝脏葡萄糖、脂肪酸代谢特点及机制。 方法 健康成年SPF级雄性SD大鼠36只,随机分为平原对照组(C组,海拔400m),高原暴露1天组(H1组)、3天组(H3组)、7天组(H7组)、15天组(H15组)、30天组(H30组)。分别测定血浆FFA、ALT、乳酸含量及血糖水平;肝脏ICDH、G6Pase、AMPK、FoxO1、CPT-I、PPARα、ACC-1的mRNA和蛋白水平以及乳酸、ATP、糖原、总酮体含量。 结果 1、与C组相比,H1、H3和H7组肝脏ICDH、G6Pase表达水平及糖原含量增高;H1组肝ATP含量减低;各组中肝AMPK和FoxO1表达水平低于C组,以H15组最低;各组肝组织乳酸含量没有明显变化。 2、与C组相比,H3组肝脏CPT-I、PPARα以及H1、H3组肝脏ACC-1表达水平明显增高,H15组明显降低;H3组大鼠肝脏酮体生成明显增加。 3、血浆ALT、FFA、乳酸含量及血糖水平无明显变化。 结论 海拔4300m高原习服大鼠在非运动状态下以有氧氧化为主,未发现无氧氧化变化的证据;以碳水化合物作为主要能源物质,脂肪酸在急性暴露期发挥补充作用;急性暴露期肝脏三羧酸循环、糖异生、糖原生成、脂肪酸氧化及合成、酮体生成增加;AMPK、FoxO1及PPARα发挥重要调节作用。
Other AbstractObjective To evaluate the characteristics and mechanisms of hepatic glucose and fatty acid (FA) metabolism in rats during high altitude (HA) acclimatization at 4300m. Methods 36 male SD rats were randomly assigned into six groups respectively named H1, H3, H7, H15 and H30 (HA exposure for 1 day, 3, 7, 15 and 30 days, respectively), and C (no HA exposure, controls). Plasma was collected to exam the content of FFA, lactate, blood glucose and ALT. The liver tissues were collected for the measurement of the mRNA and protein levels of seven factors involved in hepatic glucose and FA metabolism (ICDH, G6Pase, AMPK, FoxO1, CPT-I, PPARα and ACC-1), as well as the hepatic content of lactate, ATP, glycogen and total ketone body. Results We observed no significant difference in the blood levels of ALT, FFA, lactate and glucose as well as in the hepatic lactate content between the rats exposed to an altitude of 4,300m and those in control. Acute exposure of rats to HA was found to significantly alter the hepatic expressions of the following factors involved in glucose metabolism and FA metabolism: (1) increased levels of ICDH, G6Pase, glycogen (H1, H3, and H7) and reduced ATP content (H1); (2) decreased levels of AMPK and FoxO1 throughout the whole experimental period; (3) increased levels of CPT-I and PPARα, as well as increased production of hepatic total ketone body (H3); and (4) increased ACC-1 level (H1 and H3). Longer exposure (>15 days) caused a marked decrease in the levels of CPT-I and PPARα. Conclusions Increased hepatic gluconeogenesis, glycogen synthesis, ketogenesis, FA oxidation and synthesis in the early phase of HA exposure may be among the important mechanisms for the rats to acclimatize themselves to the stressful environments. FoxO1, PPARα and AMPK were involved in the regulation of hepatic glucose and FA metabolism.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223423
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
倪倩. 高原习服中大鼠肝脏葡萄糖、脂肪酸代谢特点及机制[D]. 兰州. 兰州大学,2014.
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