兰州大学机构库 >学院待认领
高血压性脑出血患者血肿周围组织肿瘤坏死因子-α表达与血清肿瘤坏死因子-α含量和细胞凋亡的关系
Alternative TitleRelationship between the content of serum TNF-α and the expression of TNF-αin perihematomal tissue and apoptosis in patients with hypertensive intracerebral hemorrhage
侯博儒
Thesis Advisor任海军
2010-05-20
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword脑出血 高血压 肿瘤坏死因子-α 细胞凋亡
Abstract目的 探讨高血压性脑出血患者血肿周围组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达与血清TNF-α含量和血肿周围组织细胞凋亡的关系。 方法 30例高血压性脑出血患者根据发病到手术时间随机分为<6h组、6-12h组、12-24h组、24-48h组、48-72h组、>72h组(每组n=5),各组患者临床基本情况、出血量、出血部位、手术前GCS评分及手术持续时间均无显著差异。酶联免疫吸附双抗体夹心法检测血清TNF-α含量,免疫组化法检测血肿周围组织TNF-α的表达,TUNEL染色法检测血肿周围组织凋亡细胞。 结果 高血压性脑出血血肿周围组织TNF-α阳性细胞数量和血清中TNF-α含量分别在出血后12-24h 和24-48h达高峰,分别为(57.81±6.21)个/高倍视野和(78.45±7.17 )pg /ml,且两者呈正相关( r = 0.924, P<0.05 );血肿周围脑组织TNF-α阳性细胞数量与凋亡细胞数量呈正相关( r=0.871 ,P<0.05) 。 结论高血压性脑出血患者血清TNF-α含量显著增高,血肿周围脑组织TNF-α阳性细胞数量和凋亡细胞的数量均显著增高,血清TNF-α含量与血肿周围组织TNF-α阳性细胞数量和凋亡细胞数量呈正相关。TNF-α可能与神经元凋亡有关。
Other AbstractObjective To investigate the relationship between the content of serum TNF-α and the expression of TNF-α in perihematomal brain tissue and apoptosis in patients with hypertensive intracerebral hemorrhage.Methods Thirty patients with hypertensive intracerebral hemorrhage(HICH) were randomly divided into <6 h, 6-12 h, 12-24 h, 24-48 h, 48-72 h and > 72h groups according to the time from symptom onset to surgery(n=5 in each group).There were no significant differences among the basic clinical situation ,amount of bleeding, bleeding sites,preoperative Glasgow coma scale scores and operation duration in all groups.A double-antbody sandwich enzyme-linked immunosorbent assay was used to determine the content of serum TNF-α in perihematomal tissue .Immunohistochemistry was used to detect the expression of TNF-α in perihematomal tissue ,and TUNEL staining was used to detect the apoptosis cells. Results The numbers of TNF-α positive cells and the content of serum TNF-α in perihematomal tissue reached the peaks at 12-24 h and 24-48 h respectively after intracerebral hemorrhage,respectively. There was a positive correlation between them ( r = 0.924, P < 0.05). The numbers of apoptosis cells in perihematomal tissue reached peak at 24- 48h. There was a positive correlation between the numbers of apoptosis cells and the numbers of TNF-α positive cells in perihemvenatoma ( r = 0.871, P <0.05). Conclusion The content of serum TNF-α, the numbers of TNF-α positive cells and apoptosis cells in perihemvenatomal tissue were increased significantly . The numbers of TNF-α positive cells in perihemvenatomal tissue were positive correlated with The content of serum TNF-α and he numbers of apoptosis cells in perihemvenatomal tissue. TNF-α may be related to apoptosis
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223448
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
侯博儒. 高血压性脑出血患者血肿周围组织肿瘤坏死因子-α表达与血清肿瘤坏死因子-α含量和细胞凋亡的关系[D]. 兰州. 兰州大学,2010.
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