兰州大学机构库 >学院待认领
腹腔联合注射氯胺酮及可乐定对慢性神经病理性疼痛模型大鼠的镇痛效应
Alternative TitleThe effect of Peritonea injection of clonidine and ketamine for chronic neuropathic pain model in rats
高翊博
Thesis Advisor冷玉芳
2009-05-19
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword神经病理性疼痛 脊髓背角 氯胺酮 可乐定 交感芽生 N-甲基-D-天门冬氨酸 GAP-43
Abstract目的 研究氯胺酮及可乐定对慢性神经病理性疼痛模型大鼠的镇痛作用,并探讨其可能机制。方法 雄性SD大鼠48只制作CCI模型并随机分为4组:生理盐水组(Saline)、氯胺酮组(K),可乐定组(CL)和氯胺酮+可乐定(KC)组,每组12只。Saline组腹腔注射生理盐水1ml,K组腹腔注射氯胺酮(10mg/kg);CL组腹腔注可乐定(1mg/kg);KC组腹腔注射氯胺酮(5mg/kg)+可乐定(0.5mg/kg)。各组皆于术后即刻给药,并分别于术前和术后3、7、14天测定机械缩足反射阈值和热刺激反射潜伏期,四组术后3、7、14天痛阈测试完毕后各取4只大鼠处死,取其腰段脊髓-80℃冻存测定其生长相关蛋白-43(GAP-43)mRNA的含量。结果 与术前比较,各组术后3d开始热痛阈及机械痛阈显著降低(P<0.05);与Saline组比较,K组、CL组、KC组术后7d,14d热痛阈及机械痛阈有显著升高(P<0.05),各时间点GAP-43mRNA表达明显增高(P<0.05);与术后7dK组和CL组比较,KC组的热痛阈及机械痛阈值升高较更明显(P<0.05),GAP-43mRNA表达明显降低(P<0.05)。结论 氯胺酮与一定剂量的可乐定联合应用能抑制神经病理性疼痛大鼠机械性触诱发痛和热痛觉过敏的形成,减少大鼠DRG中GAP-43mRNA的表达,具有显著的协同镇痛作用。 关键词 神经病理性疼痛;脊髓背角;氯胺酮;可乐定;交感芽生;N-甲基-D-天门冬氨酸;GAP-43
Other AbstractObjective To evaluate the analgesic effects of peritoneal injection of ketamine and clonidine in rat model of neuropathic pain. Methods 48male Sprague-Dawley rats were randomly assigned to 4 groups for varied purposes( n=12): saline group(Saline)ketamine group (K), clonidine group(CL), ketamine+clonidine(KC) group. Chronic constriction injury (CCI) of the sciatic nerve was performed in rats to form the model of neuropathic pain, with clonidine or ketamine or NS ip given immediately after the surgery. Mechanical allodynia and thermal hyperalgesia were tested before surgery and 3, 7, and 14 days afterwards. The rats were killed after the test was finished. And then we detected the GAP-43mRNA production in the spinal cord. Results in all groups,a stable mechanical allodynia and thermal hyperalgesia was formed after the surgery and become significant on 3th day(P<0.05). The combined injection of ketamine (5mg/kg) and clonidine (0.5g/kg) produced significantly more putrid analgesia than the injection of ketamine (10mg/kg) or clonidine (1mg/kg) alone. The expression of GAP-43mRNA in the KC group were significantly lower than CL and Kgroups on 3th day (P<0.05). Conclusion peritoneal injection of ketamine and clonidine can inhibit the development of mechanical allodynia and thermal hyperalgesia in the rat model of neuropathic pain, they can reduce the expression of GAP-43 mRNA in rat and can also produce synergistic abirritation. Key words neuropathic pain; DRG; ketamine; clonidine; sympathetic sproutin; NMDA; GAP-43
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223542
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
高翊博. 腹腔联合注射氯胺酮及可乐定对慢性神经病理性疼痛模型大鼠的镇痛效应[D]. 兰州. 兰州大学,2009.
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