兰州大学机构库 >学院待认领
低体积分数氧预处理对缺氧缺血性脑损伤新生大鼠脑组织脑红蛋白变化的影响
Alternative TitleEffects On The Change Of Neuroglobin With Cerebral Hypoxia-ischemia Caused by Hypoxia Preconditioning on Neonatal Rats
尹梅
Thesis Advisor金玉
2009-05-17
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword低氧预处理 新生大鼠 脑红蛋白
Abstract目的 观察低氧预处理对缺氧缺血性脑损伤(HIBD)新生大鼠脑红蛋白(Ngb)变化的影响,探讨低氧预处理与Ngb的关系。 方法 随机将出生7天Wistar大鼠120只分为三组 假手术组(n=20) 脑缺血组(HIBD组)(n=50) 低氧预处理后HIBD组(HP后HIBD组)(n=50)。HIBD组和HP后HIBD组在模型建立后1,5,15,30和60分钟各个时间点再分为五个亚组,采用双侧颈总动脉结扎的方法建立HIBD模型,应用反转录-聚合酶链反应PCR法(RT-PCR)和免疫组化的方法检测在低氧预处理干预下脑缺氧缺血后Ngb mRNA及蛋白质在不同时间的表达量。 结果 RT-PCR半定量分析:HIBD组Ngb mRNA在1,5分钟时相对表达量(A=目的基因表达量/内参表达量:1.59±0.20,1.98±0.22)分别高于假手术组(A:1.52±0.25)(P均<0.05);15,30,60分钟时表达下降(A:1.55±0.28,1.52±0.25,1.51±0.24),与假手术组相比无统计学意义(P均>0.05)。与HIBD组比较,HP后HIBD组1,5分钟时Ngb mRNA相对表达量增加(A:1.60±0.26,1.99±0.30),15,30和60分钟时下降(A:1.56±0.23,1.53±0.20,1.53±0.27),但差异均无统计学意义(P均>0.05)。免疫组化结果显示HP后HIBD组Ngb在1,5,15,30,60分钟平均光密度um(195.0±11.26,202.41±12.2,182.6±15.72,178.22±14.37,164.86±28.99)与HIBD组平均光密度um(195.59±11.59,201.4±13.61,178.09±18.92,173.75±14.3,163.45±18.66)比较无统计学意义(P均>0.05),与PCR的结果是一致的。 结论 低氧预处理对新生大鼠的脑保护作用与脑红蛋白含量的变化无相关性。
Other AbstractObjective To observe the effects of hypoxia preconditioning (HP) on the change of neuroglobin (Ngb) in neonatal rats with cerebral hypoxia-ischemia. Methods One hundred and twenty the 7-day-old wistar rats were randomly divided into 3 groups:sham operatetion group (n=20),hypoxic-ischemic brain damage (HIBD) group(n=50) and hypoxic-ischemic brain damage after hypoxia preconditioning (HP)(n=50).The rats in HIBD and HIBD after HP were further divided into 5 subgroups respectively based on 1,5,15,30,60minutes after model was set up.The neonatal rats of carotid artery ligation was used as the research model.Preconditioning was performed 24hours before hypoxic-ischemic (HIE) by placing rats in the hypoxic chamber for 3hours.The brain tissues were taken from the rats at 1,5,15,30and60min respectively after the models were made in HIBD group and HIBD after HPC group.The expressions of Ngb of brain tissues were studied at 1,5,15,30and60min after HIBD using immuno- histochemistry and quantitative reverse transcription polymerase chain reaction(RT-PCR) respectively. Result RT-PCR analysis showed that the relative expression of Ngb in brain tissue at 1,5min(A:1.59±0.20,1.98±0.22)in HIBD group were significantly higher than those in the sham-operated group(A:1.52±0.25)(Pall<0.05) respectively,but there was no significant difference at 15min,30min and 60min between HIBD group(A:1.55±0.28,1.52±0.25,1.51±0.24)and sham-operated group(Pall>0.05),and there were no significant difference between the HP group(A:1.60±0.26,1.99±0.30,1.56±0.23,1.53±0.20,1.53±0.27) and the HIBD group(Pall>0.05).The result of immunohisto- chemistry showed that there was no significant difference between the level of Ngb in HIBD after HP group(195.00±11.26,202.41±12.2,182.60±15.72,178.22±14.37,164.86±28.99) and HIBD group(195.59±11.59,201.40±13.61,178.09±18.92,173.75±14.3,163.45±18.66)(Pall>0.05). Conclusion HPC has protective effect on neonatal rats with cerebral hypoxia- ischemia,but Ngb does not have obvious protective effect on HIE.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223640
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
尹梅. 低体积分数氧预处理对缺氧缺血性脑损伤新生大鼠脑组织脑红蛋白变化的影响[D]. 兰州. 兰州大学,2009.
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