| 大黄素和替加色罗对2型糖尿病大鼠胃动力的影响 |
| Alternative Title | Effects of Emodin and Tegaserod on Gastric Dynamics in Type 2 Diabetic Rats
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| 李艳芬 |
| Thesis Advisor | 严祥
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| 2008-05-26
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 大黄素
替加色罗
胃动力
2型糖尿病大鼠
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| Abstract | 目的：观察大黄素(emodin)和替加色罗(tegaserod)对2型糖尿病大鼠模型胃动力的影响，并初步探讨其相关机制。
方法：SPF级健康雄性Wistar大鼠70只随机分为正常对照组（NOR,20只）、糖尿病模型组（MOD,50只）。以小剂量链脲佐菌素（STZ,30mg/kg）腹腔注射加长期高脂高糖饲料喂养诱导大鼠2型糖尿病模型。造模成功的大鼠随机分为4组（N＝10），即糖尿病模型对照组（MOD）、大黄素治疗组（EMO）、替加色罗治疗组（TEG）、多潘立酮治疗组（DOM），进行药物干预治疗6周。处死动物前用单光子发射计算机断层扫描技术测定胃半排空时间(GET1/2)与90分钟胃内核素残留率(RIH)。常规检测血糖、血清胰岛素、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白-胆固醇(HDL-c)，并用放免法检测大鼠血浆和胃肠组织P物质(sP) 、生长抑素(SS)的含量。
结果：模型组大鼠的胃排空较正常对照组显著减慢[GET1/2：（124.5±26.9min）vs. （63.5±13.9min）；RIH: (54.6±7.0%) vs.(40.4±6.8%), P<0.01]，而大黄素组、替加色罗组及吗丁啉组的胃排空较模型组显著加速(P<0.01 or P<0.05)。模型组血浆和胃肠组织sP较正常对照组显著降低(P<0.01)，而SS显著升高(P<0.01)。与模型组比较，替加色罗组、大黄素组及吗丁啉组的血浆和胃肠组织sP显著升高（P<0.01 or P<0.05），而SS显著降低（P<0.01 or P<0.05）。模型组、替加色罗组和多潘立酮组的血清TG和TC较正常对照组显著升高，而HDL-C显著降低(P<0.01)，但大黄素组的TG、TC和HDL-C则可以得到纠正(P<0.01)。
结论：大黄素与替加色罗能提高2型糖尿病大鼠的胃动力，而纠正部分胃肠激素的异常表达可能是其促进胃动力的机制之一。
关键词：大黄素；替加色罗；胃动力；2型糖尿病大鼠 |
| Other Abstract | Objective To investigate the effects of emodin and tegaserod on the gastrointestinal motility of type 2 diabetic rats and to explore their correlated mechanisms．
Methods Seventy SPF mail Wistar rats were randomly divided into two groups，i.e.，control(NOR) and model (MOD) groups．Murine type 2 diabetes mode1 was induced by intravenous injection of a small dose of streptozotocin plus long-term high fat high caloric laboratory chow．The rats were treated with emodin(EMO) ,tegaserod(TEG) or domperidone (DOM) for 6 weeks．Half gastric emptying time (GET1／2) and 90 min residual rate(RIH) were measured by single photon emission computed tomography before sacrifice．The blood glucose and serum insulin, triglycerides TG),total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C) were determined by biochemicaI methods．Plasma and tissue levels of substance P(sP),somatostatin(SS) were determined using radioimmunoassay.
Results Gastric emptying of type 2 diabetic rats model group was significantly slower than normal group [1／2<下标!>:(124.5±26.9min)vs. （63.5±13.9min）;RIH: (54.6±7.0%) vs.(40.4±6.8%), P<0.01], but became faster after treatment of tegaserod, emodin or domperidone（P<0.01 or P<0.05）. In comparison with normal group, plasma and intestine levels of sP in model group were significantly reduced (P<0.01),while plasma and intestine levels of SS were increased significantly (P<0.01).compared with model group，plasma and intestine levels of sP were increased in tegaserod, emodin or domperidone treated groups (P<0.01 or P<0.05), while plasma and intestine levels of SS in both groups were significantly decreased (P<0.01 or P<0.05). Serum TG and TC in the model group and tegaserod or domperidone treated groups were significantly higher while HDL-C was lower than those in normal group (P<0.01), but in emodin treated group, these detected indexes were rectified (P<0.01).
Conclusion Emodin and tegaserod can promote the gastrointestinal motility in type 2 diabetic rats．The enhancement of the gastrointestinal motility by emodin and tegaserod might be partially explained by its rectification of the abnormal expression of gastrointestinal hormones．
Key words Emodin；Tegaserod；Gastric dynamics；Type 2 diabetic rats |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223701
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
李艳芬. 大黄素和替加色罗对2型糖尿病大鼠胃动力的影响[D]. 兰州. 兰州大学,2008.
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