兰州大学机构库 >学院待认领
大黄素和替加色罗对2型糖尿病大鼠胃动力的影响
Alternative TitleEffects of Emodin and Tegaserod on Gastric Dynamics in Type 2 Diabetic Rats
李艳芬
Thesis Advisor严祥
2008-05-26
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword大黄素 替加色罗 胃动力 2型糖尿病大鼠
Abstract目的:观察大黄素(emodin)和替加色罗(tegaserod)对2型糖尿病大鼠模型胃动力的影响,并初步探讨其相关机制。 方法:SPF级健康雄性Wistar大鼠70只随机分为正常对照组(NOR,20只)、糖尿病模型组(MOD,50只)。以小剂量链脲佐菌素(STZ,30mg/kg)腹腔注射加长期高脂高糖饲料喂养诱导大鼠2型糖尿病模型。造模成功的大鼠随机分为4组(N=10),即糖尿病模型对照组(MOD)、大黄素治疗组(EMO)、替加色罗治疗组(TEG)、多潘立酮治疗组(DOM),进行药物干预治疗6周。处死动物前用单光子发射计算机断层扫描技术测定胃半排空时间(GET1/2)与90分钟胃内核素残留率(RIH)。常规检测血糖、血清胰岛素、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白-胆固醇(HDL-c),并用放免法检测大鼠血浆和胃肠组织P物质(sP) 、生长抑素(SS)的含量。 结果:模型组大鼠的胃排空较正常对照组显著减慢[GET1/2:(124.5±26.9min)vs. (63.5±13.9min);RIH: (54.6±7.0%) vs.(40.4±6.8%), P<0.01],而大黄素组、替加色罗组及吗丁啉组的胃排空较模型组显著加速(P<0.01 or P<0.05)。模型组血浆和胃肠组织sP较正常对照组显著降低(P<0.01),而SS显著升高(P<0.01)。与模型组比较,替加色罗组、大黄素组及吗丁啉组的血浆和胃肠组织sP显著升高(P<0.01 or P<0.05),而SS显著降低(P<0.01 or P<0.05)。模型组、替加色罗组和多潘立酮组的血清TG和TC较正常对照组显著升高,而HDL-C显著降低(P<0.01),但大黄素组的TG、TC和HDL-C则可以得到纠正(P<0.01)。 结论:大黄素与替加色罗能提高2型糖尿病大鼠的胃动力,而纠正部分胃肠激素的异常表达可能是其促进胃动力的机制之一。 关键词:大黄素;替加色罗;胃动力;2型糖尿病大鼠
Other AbstractObjective To investigate the effects of emodin and tegaserod on the gastrointestinal motility of type 2 diabetic rats and to explore their correlated mechanisms. Methods Seventy SPF mail Wistar rats were randomly divided into two groups,i.e.,control(NOR) and model (MOD) groups.Murine type 2 diabetes mode1 was induced by intravenous injection of a small dose of streptozotocin plus long-term high fat high caloric laboratory chow.The rats were treated with emodin(EMO) ,tegaserod(TEG) or domperidone (DOM) for 6 weeks.Half gastric emptying time (GET1/2) and 90 min residual rate(RIH) were measured by single photon emission computed tomography before sacrifice.The blood glucose and serum insulin, triglycerides TG),total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C) were determined by biochemicaI methods.Plasma and tissue levels of substance P(sP),somatostatin(SS) were determined using radioimmunoassay. Results Gastric emptying of type 2 diabetic rats model group was significantly slower than normal group [1/2<下标!>:(124.5±26.9min)vs. (63.5±13.9min);RIH: (54.6±7.0%) vs.(40.4±6.8%), P<0.01], but became faster after treatment of tegaserod, emodin or domperidone(P<0.01 or P<0.05). In comparison with normal group, plasma and intestine levels of sP in model group were significantly reduced (P<0.01),while plasma and intestine levels of SS were increased significantly (P<0.01).compared with model group,plasma and intestine levels of sP were increased in tegaserod, emodin or domperidone treated groups (P<0.01 or P<0.05), while plasma and intestine levels of SS in both groups were significantly decreased (P<0.01 or P<0.05). Serum TG and TC in the model group and tegaserod or domperidone treated groups were significantly higher while HDL-C was lower than those in normal group (P<0.01), but in emodin treated group, these detected indexes were rectified (P<0.01). Conclusion Emodin and tegaserod can promote the gastrointestinal motility in type 2 diabetic rats.The enhancement of the gastrointestinal motility by emodin and tegaserod might be partially explained by its rectification of the abnormal expression of gastrointestinal hormones. Key words Emodin;Tegaserod;Gastric dynamics;Type 2 diabetic rats
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223701
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
李艳芬. 大黄素和替加色罗对2型糖尿病大鼠胃动力的影响[D]. 兰州. 兰州大学,2008.
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