|Alternative Title||Effect of Estrogen on Cerebral Edema and Expressionof Bcl-2 and Bax of Impaired Brain Tissue after Traumatic Brain Injury in Rats
|Place of Conferral||兰州
方法:选取75只健康清洁级成年雄性 Wistar大鼠，随机分为两大组。第一组60只大鼠，用于检测伤区的脑组织Bcl-2、Bax蛋白的表达和TUNEL阳性表达。第二组15只大鼠用于检测脑含水量。每组再分为治疗组、对照组和外伤组。治疗组：用Feeney自由落体脑损伤装置致伤动物,并给予苯甲酸雌二醇(1mg/kg•d)腹腔注射治疗,于伤后立即治疗1次,以后每天治疗1次,直至动物处死；对照组：同法致伤动物，并给予等量生理盐水腹腔注射治疗；外伤组: 同法致伤动物不予治疗。等到各个时间点处死大鼠，取伤区脑组织分别进行用苏木精—伊红染色观察脑组织凋亡形态学的变化、应用免疫组织化学方法检Bcl-2及Bax阳性细胞变化并计算Bcl-2和Bax阳性细胞比值、应用TUNEL染色观察细胞凋亡并计算凋亡指数和取脑进行脑含水量测定。
|Other Abstract||Objective: To investigate whether the estrogen could protect the brain tissue or not, by influencing cerebral edema and expression of Bcl-2 and Bax of impaired brain tissue after traumatic head injury (TBI) in rats.
Methods: To select seventy-five adult male Wistar rats of health and clean, then divided into two groups stochastically. In the first group sixty rats were used to detect the expression of Bcl-2，Bax and TUNEL of impaired brain tissue. In the second group fifteen rats were used to detect the water content of brain tissues. Each group was divided into the treated group, the control group and the injury group again.The treated group: TBI model was established according to Feeney's weight-dropping model of traumatic brain injury, and peritoneal injection with benzestrofol (1mg/kg•d), immediately treated once after injury, then treated once per day, until put rat to death. The control group: the model was established by the same way, and peritoneal injection with same dose physiological saline.The injury group: the model was established by the same way but not teated. After all time point coming we put rats to death, the impaired brain tissues were stained with hematine-eosin, to observe morphologica diversity on apoptosis of neuron and detected the expression of Bcl-2 and Bax with immunohistochemistry method, then calculated the expression ratio of Bcl-2 and Bax，cell apoptosis was observed and calculated apoptosis index by TUNEL method,to obtain brain tissue then detected the water content of brain.
Results:(1) Observing HE by the light microscope: In the treated group the structure of nerve cell is more complete than the control group and the injury group, has not seen the obvious nerve cell karyopyknosis, nucleus disruption, nearby the nuclear chromatin the collection phenomenon, and the arrangement distribution is even. (2)The expression of Bcl-2 and Bax located in cytoplasm and/or around nuclear ，presented buffy. The positive cell with Bcl-2 and Bax mostly located around the injury brain tissue, less located away from injury brain tissues by light microscope. The rate of positive cell with Bcl-2 in the treated group was obviously higher than both the injury group and the control group(P<0.05)，but there was no statistics difference between the injury group and the control group(P>0.05). The percentive rate of positive cell with Bax were no statistics difference among the three group (P>0.05). The mean rate of positive cell with B...|
霍龙伟. 雌激素对大鼠颅脑创伤后伤区脑组织Bcl-2、Bax表达和脑水肿的影响[D]. 兰州. 兰州大学,2010.
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