| 吡格列酮对大鼠碱烧伤角膜IL-1α、IL-6表达的影响 |
| Alternative Title | Effect of Pioglitazone on IL-1α and IL-6 in alkali-burned corneas of rats
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| 魏海霞 |
| Thesis Advisor | 鲁建华
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| 2009-05-21
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 吡格列酮
过氧化物酶体增殖物激活受体
角膜
碱烧伤
白介素-1
白介素-6
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| Abstract | 目的：探讨过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor gamma , PPAR-γ）激动剂吡格列酮（Pioglitazone）对大鼠碱烧伤角膜中白介素-1α（IL-1α）和白介素-6（IL-6）表达的影响作用。
方法：40只SPF级Wistar大鼠随机分为5组，其中一组（Ⅰ组，n=8）作为正常对照组，其余32只大鼠均以右眼制作角膜碱烧伤模型，待大鼠苏醒后随机分为4组，每组8只。Ⅱ组为烧伤对照组，结膜下注射生理盐水，0.1ml，1次/d。Ⅲ~Ⅴ组为烧伤治疗组，分别结膜下注射 0.2g/L 、0.4g/L 、0.8g/L吡格列酮，0.1ml, 1次/d。伤后隔日裂隙灯显微镜观察角膜透明性，第7d处死大鼠，取角膜行苏木素-伊红染色，免疫组化SABC法检测IL-1α、IL-6及PPAR-γ在各组角膜组织中的表达。
结果：各治疗组角膜混浊度在各时间点均轻于烧伤对照组（P < 0.05）。烧伤对照组炎性反应明显，角膜组织中可见大量炎细胞浸润和粗大的新生血管腔，而各治疗组炎性反应减轻；与对照组相比，IL-1α、IL-6及PPAR-γ的表达在各治疗组中不同程度的下降，有统计学差异（P < 0.05）。正常对照组中仅见少量IL-1α表达，未见IL-6及PPAR-γ的表达。
结论：吡格列酮可剂量依赖性抑制碱烧伤角膜中前炎性因子的表达，减轻炎症反应。 |
| Other Abstract | Objective To investigate effect of peroxisome proliferator-activated receptor gamma ligand （Pioglitazone）on IL-1α and IL-6 in alkali-burned corneas of rats.
Methods 40 SPF level Wistar rats were divided into 5 groups randomly, one of these groups as a normal control group (Ⅰgroup, n = 8), and the remaining 32 rats were established corneal alkali-burn models in their right eyes. After corneal alkali-burn models were established, the animal models were divided into 4 groups randomly and 8 for each. 0.1ml Normal saline was subconjunctivally injected after burning of cornea in positive control group (Ⅱgroup). Pioglitazones of different level (0.2g/L 、0.4g/L 、0.8g/L) were injected at the same way in various experimental groups (Ⅲ~Ⅴgroups) respectively for once per day. Cornea opacity was assessed using a standard grading scheme in 1, 3, 5 and 7 days. Forty rats’ corneas were collected in the 7th day for histological observation and analysis by HE staining, meanwhile the levels of IL-1α, IL-6 and PPAR-γ were determined by immunohistochemistry.
Result Cornea opacity in every experimental group was superior to positive control group (P < 0.05). Inflammatory reaction was obvious in positive control group showing that massive inflammatory cells infiltration and new blood vessels formation. The expression of IL-1α, IL-6 and PPAR-γ were determined and analyzed in every group, exhibiting in every experimental group inflammatory cells infiltration was inhibited and the expression of IL-1α, IL-6 and PPAR-γ were statistically significant decreased compared with positive control group (P < 0.05). In normal control group, there was only a small amount of expression of IL-1α, and no IL-6 and PPAR-γ.
Conclusion The expression of proinflammatory cytokine in alkali-burned corneas can be inhibited by Pioglitazone in a dose-dependent manner; accordingly, corneal inflammatory reaction was downregulation. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223810
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
魏海霞. 吡格列酮对大鼠碱烧伤角膜IL-1α、IL-6表达的影响[D]. 兰州. 兰州大学,2009.
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