|Alternative Title||changes of gene expression profile of adriamycin- induced cardiomyopaathy in rats
|Place of Conferral||兰州
|Other Abstract||Objective To observe the changes of part gene expression profiles of adriamycin cardiomyopathy rats by DNA microarray.
Methods Wistar rats(n=16) were grouped randomly into a adriamycin-induced cardiomyopathy (n=8) and a normal control group(n=8). The models of adriamycin-induced cardiomyopathy in rats caused by injection of adriamycin into belly of rats were established. The cDNA probes were prepared by labeling the mRNA extracted from normal tissue and tissue of adriamycin –induced cardiomyopathy with Cy3-dUTP and Cy5-dUTP respectively through reverse transcrition. DNA microarray were constructed by spotting PCR products of 4000 rat cDNAs onto specially treated glass slides, and then hybridized against the cDNA probes followed by fluorescent signals scanning. The results were scanned and analyzed by comuputer image analysis and subjected to standardization, ratio and clustering analysis.
Results Nine hundred and twenty-three genes expressed in adriamycin-induced cardiomyopathy were screened out .Five hundred and eighty-six genes were down-regulated and three hundred and thirty-seven ones were up-regulated respectively.The expression of energy metabolism-related genes were lower in the tissues of adriamycin –induced cardiomyopathy than those of normal tissue ; The expression of apoptosis-related genes were up in the tissues of adriamycin –induced cardiomyopathy than those of normal tissue.
Conclusion Apoptosis and energy metabolism disorder may play an important role in the pathogenesis of adriamycin –induced cardiomyopathy.Investigation on the gene expression pattern is helpful for studying adriamycin –induced cardiomyopathy.
Myocardial damage is the composite results of co-variation of multiple gene changes.|
张升平. 阿霉素心肌病大鼠心肌组织部分基因表达谱的变化[D]. 兰州. 兰州大学,2005.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.