|Alternative Title||profaction of Alarelin on Cisplatin-induced Ovarian Toxicity in Famale Ret
|Place of Conferral||兰州
方法：选用清洁级、12wk雌性Wister大鼠40只（体质量245.6ｇ±15.8）随机分为A、B、C、D4组：A组给予顺铂2㎎/㎏腹腔注射，1次/d×10d； B组先给阿拉瑞林5μｇ 皮下注射，1次/d ×10d，再加顺铂2㎎/㎏腹腔注射，1次/d×共10d； C组给予阿拉瑞林5μｇ皮下注射，1次/d×20d；D组为正常对照组，给予生理盐水10 ml腹腔注射，1次/d ×10d。给药前后分别称体质量，实验结束予以心脏采血，测血清雌二醇（E2）、卵泡刺激素（FSH）水平，摘除子宫及双侧卵巢，分别称湿质量并在光镜下进行形态学和病理学观察，进行各级卵泡计数。
|Other Abstract||AIM To investigate the profection of Alarelin on ovarian toxicity in female rat undergoing Cisplatin.
METHODS We conducted an animal experiment with 12-week-old Wister rats. Forty female Wistar rats (weight 245.65ｇ±15.80) were used. They were randomly divided into four groups. The A group was used as cisplatin (2 mg/kg QD×10) administrated intraperitoneally. Group B received subcutaneously Alarelin(5μｇ QD×10 ) before simultaneously with Cisplatin(2 mg/kg QD×10) administrated intraperitoneally combination. GroupC only received Alarelin(5μｇ QD×20 ) subcutaneously.Group D was used as control with 0.9%Sodium Chloride（10ml QD×10）administrated intraperitoneally.After then blood samples were collected from rat heart determine the level of blood follicle stimulating hormaone(FSH) and estradiol(E2).Each rat was removed both ovaries and uterus to measure the amount and the maturity of follicles. weight and histomorphological feature of them were also observed.
RESULTS In comparision of the groupA to control group, weight of ovary and uterus decreased(P<0.0008)，a significant reduction in the number of each grade ovarian follicles (P<0.0008),levels of E2 were significantly lowered (P<0.0008) and levels of FSH markedly ascented in the group A.In comparison of the group B to group A,amount of primitive follicles significantly increase in the group B (P<0.0008).and Alarelin showed markedly improving effect on the ovarian and uterine morphological construction of rat.
CONCLUSION: Alarelin could improve the histomorphologic features of ovary and uterus in pathologic rat,it demonstrated that Alarelin has therapeutic effect in treating Cisplatin-induced ovarian toxicity in rat.|
郭红宇. 阿拉瑞林对顺铂所致大鼠卵巢毒性的保护作用[D]. 兰州. 兰州大学,2005.
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