| VEGF和TGF-β1在肝再生过程中的表达研究 |
| Alternative Title | Analysis of VEGF and TGF - β 1 in time-phase of rat liver regeneration
|
| 潘杰斌 |
| Thesis Advisor | 张有成
|
| 2013-05-18
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 选择性门静脉栓塞
肝再生
VEGF
TGF-β1
|
| Abstract | 目的：探讨大鼠选择性门静脉栓塞后，肝再生过程中VEGF和TGF-β1的表达变化。
方法：构建SD大鼠2/3 SPVE（portal vein embolization，SPVE）模型，分别在术后1-9天选取再生肝脏组织，留取标本。实验分组：SPVE组和正常组（未行任何处理）。在组织学及蛋白水平检测VEGF和TGF-β1在肝脏肝脏组织再生过程中的表达水平，分析VEGF和TGF-β1在肝脏中的表达与肝再生时相之间的相关性。
结果：SPVE术后，SPVE组的VEGF表达首先开始升高，约在术后第五天达到高峰，然后逐渐下降；而TGF-β1水平，上升开始较迟并且缓慢，约在第七天达到高峰，然后逐渐开始下降。正常组中VEGF和TGF-β1水平一直维持较低的水平。
结论：推测SPVE术后，VEGF与肝再生的启动过程相关，而TGF-β1与肝再生的终止过程相关。 |
| Other Abstract | Purpose: To explore VEGF and TGF-β1 expressions change in the process of liver regeneration after selective portal vein embolization of rats(SPVE).
Methods: Two-thirds SPVE model was constructed by a embolization of left portal vein and collected regenerative liver tissues in 1-9 days, postoperative and respectively. 50 of SD rats were divided into group SPVE ( 45 of rats) and group control (5 of rats ). The expressions of VEGF and TGF-β1 in the liver regeneration tissues were detected with Immunohistochemistry and western blot, and the relationship of expressions of VEGF/TGF-β1 and time-phase of rat liver regeneration was analyzed.
Results: In group SPVE compared to the group control, the expression of VEGF increased during day 1 to day 5 after SPVE procedure, with a peak level at day 5, and the expressions of TGF-β1 increased during day 3 to day 7 after SPVE procedure, with a peak level at day 7, and a gradual decrease was appeared from the day to the peak.
Conclusions: The results implied that the VEGF fluctuation may correlate with the start of hepatic regeneration, while the termination of hepatic regeneration may be along with the TGF-β1 fluctuation after SPVE. |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/223874
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
潘杰斌. VEGF和TGF-β1在肝再生过程中的表达研究[D]. 兰州. 兰州大学,2013.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
