| TGF-α和p63在男性尖锐湿疣及阴茎癌组织中表达的研究 |
| Alternative Title | Expressive study of TGF-α and p63 in the lesions of male condyloma
|
| 张嫦娥 |
| Thesis Advisor | 李文竹
|
| 2009-05-26
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 尖锐湿疣
阴茎癌
人类乳头瘤病毒
转化生长因子-α
p63
|
| Abstract | 【第一部分】 目的： 探讨转化生长因子（TGF）-α 和p63在尖锐湿疣(CA)发病机制中的作用。方法：用聚合酶链反应（PCR）技术对34例男性CA组织进行检测；将有HPV6/11型感染的CA组织和正常包皮组织15例进行免疫组化（SP）法染色。结果：34例CA标本中有26例HPV 6/11型感染，阳性率为76.92%；CA组织中TGF-a和p63阳性表达率均为92.31％(24/26)。TGF-α和p63的表达强度在CA和正常包皮组织之间均有统计学意义（P<0.05）；TGF-α和p63间有相关性（相关系数 rs=0.556, P=0.003<0.05）。结论: CA感染中以HPV6/11型占多数；TGF-α和p63在CA发病中起一定作用。
【第二部分】 目的: 研究TGF-α和p63在阴茎癌(PC)发病机制中的作用。方法：用PCR技术对26例PC组织进行检测；将有HPV感染的PC组织和正常包皮组织15例进行免疫组化（SP法）染色。结果：26例PC标本中有20例HPV DNA阳性，阳性率为76.47%；PC组织中TGF-a和p63阳性表达率分别为90％(18/20)，95%（19/20）。TGF-α和p63的表达程度在PC和正常包皮组织之间均有统计学意义（P<0.05）；TGF-α和p63间有相关性（相关系数 rs=0.467, P=0.038<0.05）。结论: PC中以HPV感染占多数；TGF-α和p63在PC发病中起一定作用。 |
| Other Abstract | 【Part Ⅰ】 Objective: To investigate the roles of transforming growth factor –alpha (TGF–α)and P63 in pathogenesis of Condyloma acuminatum(CA). Methods: Polymerase Chain Reaction（PCR）used to detect the infection of HPV 6/11 type in 34 male cases with CA ; Immunohistochemical (streptavidin /peroxidase SP) method tested the expressions of TGF-α and p63 both in CA lesions with HPV 6/11 type and in normal prepuce of 15 cases. Results: There were 26 positive specimens with HPV 6/11 type in 34 lesions, the detection rate was 76.47% (26/34); The positive rates of both TGF-α and p63 were 92.31% (24/26). There were statistical differences in the detections of both TGF-α and P63 between in CA and in normal prepuce （P<0.05）; Spearman related analysis between TGF-a and p63 was correlation,（correlation coefficient rs=0.556, P=0.003<0.05）. Conclusions: The infection of HPV 6/11 type accounts for majority in CA ; And TGF-α and p63 play certain roles in the development of CA．
【Part Ⅱ】 Objective: To approach the roles of TGF –α and p63 in pathogenesis of Penile carcinoma (PC). Methods: PCR used to detect the infection of HPV in 26 cases with PC ; Immunohistochemitry (SP) tested the expressions of TGF-α and p63 both in PC with HPV infections and in normal prepuce of 15 cases. Results: There were 20 positive specimens with HPV infections in 26 lesions, the detection rate was 76.92% (20/26); The positive rates of both TGF-α and p63 were 90% (18/20) and 95% (19/20) respectively. There were statistical differences in the expressions of both TGF-α and p63 between in PC and in normal prepuce（P<0.05）; Spearman related analysis between TGF-a and p63 was correlation,（correlation coefficient rs=0.467, P=0.038<0.05）. Conclusions: The infection of HPV accounts for majority in PC ; And TGF-α and p63 play certain roles in the development of PC． |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/223900
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
张嫦娥. TGF-α和p63在男性尖锐湿疣及阴茎癌组织中表达的研究[D]. 兰州. 兰州大学,2009.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
