兰州大学机构库 >学院待认领
SAHA预处理对初进高原致死性失血性休克大鼠的救治作用及肺保护作用的研究
Alternative TitleStudy on the therapeutic and lung protective effect of SAHA pretreatment of lethal hemorrhagic shock on rats first arrival at high altitude
张彦圆
Thesis Advisor耿智隆
2013-05-21
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword致死性失血性休克 SAHA 初进高原 生存率 肺保护
Abstract目的 探讨辛二酰苯胺异羟肟酸(Suberoylanilide Hydroxamic Acid ,SAHA)预处理对初进高原致死性失血性休克大鼠早期生存率的影响。 方法 36只初进高原雄性SD大鼠(体重=260±20 g),采用随机数字法分为3组(n=12):假手术组(Sham组),生理盐水(Normal Saline,NS)预处理组(NS组),SAHA预处理组(SAHA组)。Sham组麻醉后单纯手术操作,不给予失血处理; NS组和SAHA组于实验前24 h和失血前经皮下分别给予0.25 ml NS或溶于等量NS的SAHA(7.5 mg/kg)溶液预处理,制备初进高原大鼠致死性失血性休克模型后,各组持续监测平均动脉压(MAP)至休克后60 min。失血前后及休克后60 min三个时间点行动脉血气分析。观察大鼠180 min,记录每只大鼠死亡时间,判断大鼠60 min、120 min和180 min生存率。 结果 SAHA组大鼠休克后60 min、120 min和180 min生存率均显著高于NS组(P<0.05)。休克15 min到60 min,与NS组比较,SAHA组大鼠休克后MAP、动脉血PH、PaO2和BE值显著升高(P<0.05),Lac浓度显著降低(P<0.05),而PaCO2无明显变化。 结论 SAHA预处理可显著提高初进高原致死性失血性休克大鼠早期生存率,维持失血后血流动力学的相对稳定。
Other AbstractObjective To investigate the therapeutic effect of SAHA pretreatment of lethal hemorrhagic shock on first arrival at high altitude in rats. Methods 32 male SD rats were randomly divided into three groups: Sham, NS and SAHA. Rats of group Sham were surgically manipulated but not hemorrhaged. Rats of other groups were reproduced as models of lethal hemorrhagic shock in rats first entering high altitude. Rats of group NS and group SAHA were administered subcutaneously with NS or SAHA, 24 hours and immediately before lethal hemorrhaged . MAP were monitored constantly. Arterial blood gas were measured at baseline, end of hemorrhage and 60 min after shock. Rats were observed 180 min, record death time point of every rats, decide survival rates of 60 min ,120 min and 180 min of each group. Results Group SAHA got a higher survival rates of 60 min ,120 min and 180 min (P<0.05).The MAP in SAHA group was higher than NS group from 15 min to 60 min. Pretreated with SAHA increased arterial blood levels of PH, PaO2 and BE, decreased levels of lactic acid (P<0.05). Conclusion Pretreatment with SAHA increases early survival rates first arrival at high altitude suffered lethal hemorrhagic shock .
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223943
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
张彦圆. SAHA预处理对初进高原致死性失血性休克大鼠的救治作用及肺保护作用的研究[D]. 兰州. 兰州大学,2013.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[张彦圆]'s Articles
Baidu academic
Similar articles in Baidu academic
[张彦圆]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[张彦圆]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.