兰州大学机构库 >学院待认领
PTEN、PI3K、AKT2和NF-κB的表达与胃癌生物学行为的关系
Alternative TitleRelationship between expression of PTEN、PI3K、AKT2、NF-κB in gastric carcinoma and its biological behavior
邹绍静
Thesis Advisor周永宁
2009-05-22
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword胃癌 PTEN PI3K AKT2 NF-κB 临床病理学 生存期
Abstract目的 研究胃癌组织中PTEN、PI3K、AKT2、NF-κB蛋白的表达,探讨其表达与临床病理特征及生存期的关系。 方法 189例胃癌手术切除标本,54例癌旁组织及41例胃癌淋巴结制作成组织芯片。32例正常胃粘膜作为对照。应用免疫组织化学S-P法及组织芯片技术检测PTEN、PI3K、AKT2 、NF-κB蛋白的表达。对有生存资料的101例胃癌患者进行Kaplan-Meier生存分析,并应用Cox比例风险回归模型分析预后因素。 结果 1.胃癌、癌旁和对照组PTEN阳性表达率差异具有统计学意义(χ2=37.456,P=0.000)。胃癌组织PTEN表达与有无淋巴结转移、分化程度和Lauren分型有关(P<0.05)。2.胃癌、癌旁和对照组PI3K阳性表达率的差异具有统计学意义(χ2=64.817,P=0.000)。胃癌组织PI3K表达与有无淋巴结转移、浸润深度、分化程度和Lauren分型有关(P<0.05)。3.胃癌、癌旁和对照组AKT2阳性表达率的差异具有统计学意义(χ2=56.518,P=0.000)。胃癌组织AKT2表达与肿瘤大小、有无淋巴结转移、浸润深度、分化程度、Lauren分型有关(P<0.05)。4.胃癌、癌旁和对照组NF-κB阳性表达率的差异具有统计学意义(χ2=51.680,P=0.000)。胃癌组织NF-κB表达与有无淋巴结转移、浸润深度、分化程度、Lauren分型有关(P<0.05)。5.41例淋巴结转移灶和相对应的41例胃癌中的表达:两组间PTEN阳性表达率分别为29.3%、63.4%,差异具有统计学意义(χ2=9.612,P=0.002)。两组间NF-κB阳性表达率分别为90.2%、70.7%,差异有统计学意义(χ2=4.970,P=0.026)。两组间PI3K阳性表达率分别为85.4%、75.6%,差异无统计学意义(χ2=1.242,P=0.265)。两组间AKT2阳性表达率分别为80.5%、75.6%,差异无统计学意义(χ2=0.285,P=0.594)。6Kaplan-Meier生存分析:对有生存资料的101例胃癌病人进行生存分析,PTEN阳性、PI3K阴性、AKT22阴性、NF-κB阴性组生存期分别高于PTEN阴性组、PI3K阳性、AKT2阳性和NF-κB阳性组(P<0.05)。无淋巴结转移组生存期高于有淋巴结转移组(χ2=13.007,P=0.458)。浸润深度未及浆膜层组生存期高于浸润深度达浆膜及浆膜以外组(χ2=20.60,P=0.000)。高/中分化生存率高于低分化组(χ2=4.836,P=0.028)。Φ<5cm组生存率高于Φ≥5cm组(χ2=4.224,P=0.000)。肠型胃癌生存率高于弥漫型胃癌生存率(χ2=5.263,P=0.022)。 结论:1.胃癌组织中存在PI3K、AKT2、NF-κB蛋白表达上调,PTEN蛋白表达下调。表明PTEN、PI3K、AKT2 、NF-κB参与了胃癌的发生发展。2.上调的NF-κB可能是胃癌发生的早期事件,表达下调的PTEN和上调PI3K、AKT2可能是胃癌发生的晚期事件。3. PTEN、AKT2、NF-κB、淋巴结转移和浸润深度是胃癌独立的预后因素。 关键词: 胃癌;PTEN;PI3K;AKT2;NF-κB;临床病理学;生存期
Other AbstractObjective: To study the expression of PTEN, PI3K, AKT2 and NF-κB in gastric carcinoma and analyze the features of them and the relationship between them with clinicopathology as well as patient prognosis. Methods: 189 cases of gastric carcinoma, 54 cases of paracancer and 56 cases of lymph nodes were made into tissue microarray. Meanwhile 32 cases of normal gastric tissue were chosen as control. PTEN, PI3K, AKT2 and NF-κB were detected by immunohistrochemistry staining. Kaplan-Meier survival analysis was performed for 101 cases with survival time. We analyzed the prognostic factors by Cox regression model in these cases. Results: 1. The positive rates for PTEN of the gastric carcinoma, paracancer and control were respectively 50.8%, 85.2% and 96.9%, with significant difference (P < 0.05) among the three case groups. The positive rates for PTEN of the positive lymph node metastasis and without lymph node metastasis were 43.1% and 63.0% respectively. There was significant difference between the two groups(χ2=7.106,P=0.008). The positive rates for PTEN of the differentiated gastric carcinoma and undifferentiated gastric carcinoma were 60.7% and 42.0%respectively. There was significant difference between the two groups(χ2=6.570,P=0.010). The positive rate for PTEN of intestinal gastric carcinoma was higher than that of diffused gastric carcinoma (χ2=7.101,P=0.008). 2.The positive rates for PI3K of the gastric carcinoma, paracancer and control were respectively 76.7%、25.9% and 25.0%, with significant difference (P < 0.05) among the three case groups. The positive rates for PI3K of the positive lymph node metastasis and without lymph node metastasis were 82.8% and 67.1% respectively. There was significant difference between the two groups(χ2=6.132,P=0.013), The positive rates for PI3Kof the gastric carcinoma with invasion under serosa and that with invasion out of serosa were respectively 65.2% and 80.4%, the former was higher than the latter (χ2=4.503, P=0.034). The positives rates for PI3K of the differentiated gastric carcinoma and undifferentiated gastric carcinoma were 68.5% and 84.0% respectively. There was significant difference between the two groups(χ2=6.302,P=0.012). The positive rate for PI3K of diffused gastric carcinoma was higher than that of intestinal gastric carcinoma (χ2=4.526,P=0.033). 3.The positive rates for AKT2 of the gastric carcinoma, paracancer and control were 75.7%、27.8% and 37...
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223961
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
邹绍静. PTEN、PI3K、AKT2和NF-κB的表达与胃癌生物学行为的关系[D]. 兰州. 兰州大学,2009.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[邹绍静]'s Articles
Baidu academic
Similar articles in Baidu academic
[邹绍静]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[邹绍静]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.