兰州大学机构库 >学院待认领
PSCA、PLCE1、PRKAA1基因多态性及幽门螺旋杆菌感染与胃癌易感性的关系
Alternative TitleAssociation of Helicobacter pylori infection and Polymorphisms of PSCA, PLCE1 and PRKAA1 with Gastric Cancer Susceptibility
吴迪
Thesis Advisor李玉民
2014-05-26
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword胃癌 基因多态性 PSCA PLCE1 PRKAA1 幽门螺旋杆菌
Abstract目的:通过检测甘肃河西地区汉族中胃癌、癌前病变和正常对照人群PSCA(rs2294008)、PLCE1(rs3203713、rs11599672)和PRKAA1(rs13361707)基因多态性及幽门螺旋杆菌(Helicobacter pylori,H. pylori)感染,探讨其与胃癌易感性的关系。 方法:根据实验设计将研究对象分为三组,共计747例,其中胃癌组217例,癌前病变组102例,正常对照组428例,所有病例均经组织病理学确诊。采集病例的性别、年龄、胃癌家族史和流行病学等资料。采用多重单碱基延伸法(Multiplex SNaPshot)进行SNP分型;采用尿素[13C]呼气试验检测H.pylori感染。数据统计采用单因素方差分析、X2检验和logistic回归进行统计分析。 结果: 携带rs2294008 CT/TT基因型者胃癌发生的风险是CC基因型的1.778倍(OR,1.778; 95%CI,1.272~2.484; P =0.001);rs11599672 AC/CC基因型携带者患胃癌的风险是AA基因型的1.981倍(OR,1.981; 95%CI, 1.423~2.758; P =0.000),癌前病变恶变潜能是AA型携带者的1.960倍(OR,1.960; 95%CI, 1.216~3.159; P=0.005);rs13361707 CT/CC基因型携带者患胃癌的风险是TT基因型的1.587倍(OR,1.587; 95%CI, 1.085~2.322; P =0.017)。通过logistic回归分析发现,rs2294008 CT/TT基因型、rs11599672 AC/CC基因型、rs13361707 CT/CC基因型、饮酒、胃癌家族史和H.pylori感染都与胃癌的发病风险有关,而且它们在胃癌的发病风险中都存在相加的交互作用;胃癌家族史与rs11599672 AC/CC基因型与癌前病变的恶变潜能有关,并且二者共同作用时增加癌前病变的恶变潜能;同时携带rs2294008 CT/TT、rs11599672 AC/CC和rs13361707 CT/CC基因型的个体患胃癌的风险是rs2294008 CC、rs11599672 AA和rs13361707 TT基因型携带者的6.792倍(OR,6.792;95%CI, 2.521~18.296)。 结论:PSCA(rs2294008)、PLCE1(rs11599672)、PRKAA1(rs13361707)基因多态性与饮酒、胃癌家族史及H. pylori感染等因素和中国甘肃地区汉族人群胃癌发病风险可能有关。
Other AbstractObjective: By testing the polymorphism of PSCA(rs2294008), PLCE1 ( rs3203713、rs11599672 ) and PRKAA1(rs13361707)and Helicobacter pylori infection among gastric cancer, precancerous and normal control group in Gansu Han population, to investigate the association of them with gastric cancer susceptibility. Methods: A total of 747 cases were divided into three groups, including 217 gastric cancers, 102 precancerous and 428 normal controls, all cases were diagnosed by pathology. Clinical datas were collected including gender, age, family history of gastric cancer, epidemiological data etc.. The tests of SNP types were used by the method of multiplex single base extension (Multiplex SNaPshot). Detection of H. pylori infection was used by urea [C13] breath test. The data was analysed through X2 test, single factor variance analysis and logistic regression. Results: We found that the risk of gastric cancer for rs2294008 CT/TT genotype carriers was 1.778 times higher than that of CC genotype carriers (OR, 1.778; 95%CI, 1.272~2.484; P=0.001); rs11599672 AC/CC genotype carriers 1.981 times higher than AA genotype carriers (OR, 1.981; 95%CI, 1.423 ~2.758; P =0.000) and the malignant potential of precancerous lesions 1.960 times higher than AA genotype carriers (OR,1.960; 95%CI, 1.216~3.159; P=0.005);rs13361707 CT/CC genotype carriers 1.587 times higher than TT genotype (OR, 1.587; 95%CI, 1.085~2.322; P=0.017). Logistic regression analysis showed that rs2294008 CT/TT genotype , rs11599672 AC/CC genotype, rs13361707 CT/CC genotype, drinking, family history of gastric cancer and H. pylori infection were associaed with the risk of gastric cancer, and there were additive interaction for the risk of gastric cancer between these three kinds of genotypes and alcohol drinking, family history of gastric cancer and H. pylori infection. Both the genotype of rs11599672 AC/CC and positive family history were associated with precancerous lesions of the malignant potential, and the two together could make the precancerous lesions of the malignant potential increase. The risk of gastric cancer was 6.792 times higher compared individuals who carried rs2294008 CT/TT, rs11599672 AC/CC and rs13361707 CT/CC genotype simultaneously with rs2294008 CC, rs11599672 AA and rs13361707 TT genotype carriers (OR, 6.792; 95%CI, 2.521~18.296). Conclusion:There may be an associton between PSCA (rs2294008), PLCE1 (rs11599672), PRKAA1 (rs13361707) gene polymorphism and alcohol dr...
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223964
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
吴迪. PSCA、PLCE1、PRKAA1基因多态性及幽门螺旋杆菌感染与胃癌易感性的关系[D]. 兰州. 兰州大学,2014.
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