兰州大学机构库 >学院待认领
NanogmRNA在骨肉瘤类肿瘤干细胞中的表达和意义
Alternative TitleSignification of Nanog gene expression in tumor-like stem cell of osteosarcoma
韩兴文
Thesis Advisor王栓科
2009-05-22
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword肿瘤干细胞 骨肉瘤 Nanog基因 骨肉瘤类肿瘤干细胞
Abstract目的:观察骨肉瘤类肿瘤干细胞中Nanog基因的表达和意义。方法:用无血清培养法从骨肉瘤细胞株U2-OS、MG-63中分离培养悬浮细胞球,免疫磁珠分选Stro-1阳性细胞,反转录-聚合酶链反应(RT-PCR)法检测Stro-1阳性细胞、Stro-1阴性细胞及悬浮生长细胞中Nanog mRNA的表达。结果:Stro-1阳性细胞和悬浮细胞Nanog mRNA表达水平1.69±0.14, 1.56±0.18,均高于Stro-1阴性细胞的表达水平0.19±0.35(P<0.05)。随着培养时间的延长悬浮细胞球Nanog mRNA的表达比早期悬浮细胞球有所下降(P<0.05)。结论:骨肉瘤类肿瘤干细胞高度表达Nanog-mRNA,该基因可能有助于骨肉瘤类肿瘤干细胞自我更新和维持其未分化状态。
Other AbstractAIM: To study the expression of Nanog gene in tumor-like stem cells of osteosarcoma and its signification.METHODS:Tumor sphere cells were isolated from osteosarcoma cell line U2-OS and MG-63 with serum-free culturing techniques,Stro+ cells were isolated using magnetic activated cell sorting system,RT-PCR was used to detect the expression of Nanog gene in Stro-1+ cells,Stro-1- cells and tumor sphere cells. RESULTS:The level of Nanog mRNA expression in stro-1+ cells and tumor sphere cells 1.69±0.14,1.56±0.18 higher than the Stro-1- cells(P<0.05).With the extension of culture expression of Nanog mRNA in sphere cells were lower than the ealier time(P<0.05)CONCLUSION:Over expression of Nanog gene was observed in Osteosarcoma-like stem cells,this gene might have conduced to sustain the self-renewal and the undifferentiation of osteosarcoma-like stem cell.
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224024
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
韩兴文. NanogmRNA在骨肉瘤类肿瘤干细胞中的表达和意义[D]. 兰州. 兰州大学,2009.
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