兰州大学机构库 >学院待认领
MiR-34a与MiR-125b在类风湿性关节炎患者外周血单个核细胞中的表达及临床意义
Alternative TitleExpression of miR-34a and miR-125b in peripheral blood mononuclear cells of patients with rheumatoid arthritis and clinical significance
刘玮玮
Thesis Advisor李光迪
2015-05-21
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword类风湿关节炎 miR-34a miR-125b ESR CRP
Abstract目的:对miR-34a 与miR-125b分别在类风湿性关节炎(RA)、骨性关节炎(OA)及正常对照人群外周血单个核细胞(PBMCs)中的表达情况进行研究,从而进一步探究在类风湿性关节炎的发病机制中miR-34a、miR-125b所起的作用,寻找能够早期诊断类风湿性关节炎的分子标志物,以及卓有成效的药物治疗靶点,降低类风湿关节炎患者的疾病致残率,提高患者的生活质量。 方法:选取RA患者35例、OA患者15例、健康对照者20例作为研究对象。分别留取各组标本外周血,用淋巴细胞分离液分离出PBMCs,提取总RNA,应用实时荧光定量聚合酶链反应(RT-qPCR),检测miR-34a、miR-125b在PBMCs中的相对表达水平,并通过结合常规诊断类风湿性关节炎的血清标志物ESR、CRP、CCP、RF的表达情况进行比对,进一步分析相关microRNAs与疾病内在的发病机理的相关性。 结果:1. RA组患者PMBCs中miR-34a、miR-125b表达水平均低于健康对照组(P均<0.05);2. OA组PBMCs中miR-34a表达水平与健康对照组相比没有显著性差异(P>0.05);而在OA组中miR-125b的表达水平低于健康对照组(P<0.05);3. miR-34a及miR-125b的表达水平与ESR、CRP呈正相关(P均<0.05),与RF呈负相关(P均>0.05),与CCP抗体无显著相关性。 结论:RA患者PBMCs中miR-34a、miR-125b的表达水平均下调,提示miR-34a及miR-125b可能参与了RA的发生发展。
Other AbstractObjective: To investigate the expression of miR-34a and miR-125b in the peripheral blood mononuclear cell(PBMCs) of patients with rheumatoid arthritis(RA), Osteoarthritis(OA), to further explore the role of miR-34a and miR-125b in the pathogenesis of RA,to find out the marker which can dagnose RA early,and effective drug treatment targets, reduce the disease morbidity in patients with rheumatoid arthritis, to improve the patient''''s quality of life. Methods: There were 35 patients with RA, 15 patients with OA and 20 healthy controls as the research object. PBMCs were isolated from peripheral blood in using Lymphocytes Separation Medium, which was used to extract total RNA, the expression levels of miR-34a and miR-125b were measured using real-time RT-PCR,The results combined with the clinic data of patients were analysed. Results: The levels of miR-34a and miR-125b were significantly lower in the PBMCs from RA patients than healthy individuals and OAgroup (P﹤0.05).The expression of miR-125b were significant lower between OA groups and healthy individuals (P﹤0.05).The expression levels of miR-34a in RA patients were positively correlated to CRP, were negatively correlated to RF, but not to ESR and anti-CCP,The levels of miR-125b were positively correlated to CRP and ESR,were negatively correlated to RF, but not to anti-CCP. The expression levels of miR-34a and miR-125b in OA patients were positively correlated to CRP and ESR, but not to RF and anti-CCP.This suggests the expression of miR-34aand miR-125b may be related with the disease activity of RA and OA. Conclusion: The expression levels of miR-34a and miR-125b were significantly lower in the PBMCs of RA, and were associated with the laboratory indexes of RA, Its expression level might be related to RA occurrence, development and transformation, To explore the role of miR-34a and miR-125b in rheumatoid arthritis and the possible mechanism for help.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224046
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
刘玮玮. MiR-34a与MiR-125b在类风湿性关节炎患者外周血单个核细胞中的表达及临床意义[D]. 兰州. 兰州大学,2015.
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