|Alternative Title||Expression of miR-34a and miR-125b in peripheral blood mononuclear cells of patients with rheumatoid arthritis and clinical significance
|Place of Conferral||兰州
结果：1. RA组患者PMBCs中miR-34a、miR-125b表达水平均低于健康对照组（P均＜0.05）；2. OA组PBMCs中miR-34a表达水平与健康对照组相比没有显著性差异（P＞0.05）；而在OA组中miR-125b的表达水平低于健康对照组（P＜0.05）；3. miR-34a及miR-125b的表达水平与ESR、CRP呈正相关（P均＜0.05），与RF呈负相关（P均＞0.05），与CCP抗体无显著相关性。
|Other Abstract||Objective: To investigate the expression of miR-34a and miR-125b in the peripheral blood mononuclear cell(PBMCs) of patients with rheumatoid arthritis(RA), Osteoarthritis(OA), to further explore the role of miR-34a and miR-125b in the pathogenesis of RA,to find out the marker which can dagnose RA early,and effective drug treatment targets, reduce the disease morbidity in patients with rheumatoid arthritis, to improve the patient''''s quality of life.
Methods: There were 35 patients with RA, 15 patients with OA and 20 healthy controls as the research object. PBMCs were isolated from peripheral blood in using Lymphocytes Separation Medium, which was used to extract total RNA, the expression levels of miR-34a and miR-125b were measured using real-time RT-PCR,The results combined with the clinic data of patients were analysed.
Results: The levels of miR-34a and miR-125b were significantly lower in the PBMCs from RA patients than healthy individuals and OAgroup (P﹤0.05).The expression of miR-125b were significant lower between OA groups and healthy individuals (P﹤0.05).The expression levels of miR-34a in RA patients were positively correlated to CRP, were negatively correlated to RF, but not to ESR and anti-CCP,The levels of miR-125b were positively correlated to CRP and ESR,were negatively correlated to RF, but not to anti-CCP. The expression levels of miR-34a and miR-125b in OA patients were positively correlated to CRP and ESR, but not to RF and anti-CCP.This suggests the expression of miR-34aand miR-125b may be related with the disease activity of RA and OA.
Conclusion: The expression levels of miR-34a and miR-125b were significantly lower in the PBMCs of RA, and were associated with the laboratory indexes of RA, Its expression level might be related to RA occurrence, development and transformation, To explore the role of miR-34a and miR-125b in rheumatoid arthritis and the possible mechanism for help.|
刘玮玮. MiR-34a与MiR-125b在类风湿性关节炎患者外周血单个核细胞中的表达及临床意义[D]. 兰州. 兰州大学,2015.
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