兰州大学机构库 >学院待认领
MCM5和P16INK4A在宫颈鳞癌和癌前病变中的表达及其临床意义
Alternative TitleExpression and Clinical Significance of MCM5 and P16INK4A in Cervical Squamous Cell Carcinomas and Precancerous Disease
刘丽娜
Thesis Advisor杨永秀
2014-05-20
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword微小染色体维持蛋白5 P16INK4A 宫颈鳞癌 免疫组化 实时荧光定量PCR
Abstract目的:通过检测微小染色体维持蛋白-5(MCM5)与P16INK4A在宫颈鳞癌、宫颈上皮内瘤变(CIN)及正常宫颈组织中的表达情况,分析二者与宫颈鳞癌的临床病理学特征的关系及二者在宫颈鳞癌中相关性,探究二者在宫颈癌变过程中的作用及临床意义。 方法:采用免疫组织化学SABC法和实时荧光定量PCR方法,分别检测MCM5和P16INK4A mRNA及蛋白在40例宫颈鳞癌、11例CINⅠ、15例CINⅡ-Ⅲ和15例正常宫颈组织中的表达。 结果:1. MCM5和 P16INK4AmRNA的表达量随着宫颈病变程度的增加而逐渐升高。MCM5基因在宫颈癌、CINⅠ、CINⅡ-Ⅲ组织中的表达量高于正常宫颈组织中的表达量,差异具有统计学意义;P16INK4A基因在宫颈癌组与正常组、低级宫颈上皮内瘤变组比较,具有统计学差异。在正常宫颈、CINⅠ、CINⅡ-Ⅲ、宫颈鳞癌组织中MCM5和P16INK4A的阳性表达率分别为:20%、63.6%、73.3%、97.5%;0%、27.3%、86.7%、95.0%。2.MCM5 mRNA表达量和蛋白表达与临床期别、分化程度相关;与年龄无相关性;P16INK4A mRNA表达量和蛋白表达与临床期别、年龄无相关性;与分化程度相关。宫颈鳞癌中,MCM5和P16INK4A的表达显示为正相关。 结论:MCM5、P16INK4A的表达随着宫颈病变程度的进展逐渐增加;在宫颈鳞癌组织中,MCM5、P16INK4A的异常表达与肿瘤分化程度有关。有望成为宫颈癌前病变与宫颈癌的有效生物标记物。
Other AbstractObject: To investigate the expression of MCM5 and P16INK4A in cervical tissues of differential pathological types, especially in cervical intraepithelial neoplasia(CIN) and cervical carcinomas, in order to analyse the relationship of MCM5 and P16INK4A with clinical and pathological features of cervical carcinoma and the relevance of each other. Methods: Real-time fluorescence quantitative PCR and immunohistochemical staining SABC method were used respectively to examine the expression of mRNA and protein of MCM5 and P16INK4A in 40 cases of cervical squamous cell carcinoma, 11 cases of CINⅠ, 15 cases of CINⅡ-Ⅲ and 15 cases of normal cervical tissues. The expression of of mRNA and protein of MCM5 and P16INK4A was compared in different cervical tissues. Results: 1. The expression of MCM5 and P16INK4A mRNA added with the progress of cervical lesion. The expression of mRNA of MCM5 was significantly up-regulated in carcinomas, CINⅠ and CINⅡ-Ⅲ than normal cervical tissues, there were significant difference with each other . There were significant difference when the group of cervical cancer compared with the group of CINⅠ and normal cervical tissues . MCM5 and P16INK4A positive expression rates in 15 normal cervical tissue,11 CINⅠ,15 CINⅡ-Ⅲ and 40 cervical squamous cell carcinoma samples were 20%, 63.6%, 73.3%, 97.5% , 0%、27.3%、86.7%、95.0% respectively. 2. there was association between MCM5 expression and clinical stage, the differentiation. however, there was no association between MCM5 expression and age. there was association between P16INK4A expression and the differentiation of cervical cancer, but the expression of P16INK4A showed no significant relation in different age and clinical stage. In invasive cervical cancer, the expression of MCM5 and P16INK4A showed positive correlation. Conclusion: The expression of MCM5 and P16INK4A increased with the progress of cervical lesion. The expression of MCM5 and P16INK4A had correlation with the grades of histological in cervical squamous carcinoma. There was an important meaning of predicting outcome of cervical intraepithelial neoplasia and the progression and prognosis of cervical cancer by union examining MCM5 and P16INK4A .
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224059
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
刘丽娜. MCM5和P16INK4A在宫颈鳞癌和癌前病变中的表达及其临床意义[D]. 兰州. 兰州大学,2014.
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