兰州大学机构库 >学院待认领
IL-10基因多态性与环境因素在甘肃河西地区胃癌高发中交互作用的研究
Alternative TitleInteraction of IL-10 Gene Polymorphism and environmental factor to Gastric
曾祥挺
Thesis Advisor李玉民
2010-05-19
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword胃癌 白细胞介素10 基因多态性 幽门螺旋杆菌
Abstract中文摘要 目的:通过测定甘肃河西地区人群胃癌、癌前病变及正常对照组白细胞介素10( interleukin 10, IL-10)基因启动子-1082位点基因多态性及幽门螺旋杆菌(Helicobacter pylori,HP)的分型,了解该位点在河西地区人群胃癌、癌前病变及正常对照组健康人群中的基因型频率、等位基因频率的分布特征,及HP不同分型的资料,探讨IL-10-1082位点的单核苷酸多态性、不同类型HP感染与胃癌易感性之间的关系。 方法: 根据实验设计将试验对象分为三组,胃癌组和癌前病变组为经病理确诊的原发性胃癌151例、癌前病变148例, 153例健康人组成的对照组。收集病人的临床资料,包括年龄、性别、流行病学资料、胃癌家族史、胃镜或术后诊断、病理结果。采用SNaPshot SNP分型技术检测151例胃癌患者、148例胃癌癌前病变患者以及153例健康人群的外周血标本IL-10基因-1082位点的多态性;并用HP分型试剂盒对研究对象幽门螺杆菌( Hp)感染情况进行分型检测。 结果: ①对照人群IL-10 -1082位点AA、AG/GG 2 种基因型分布频率分别为75.8%、24.2%;HP感染情况为HPⅠ型46.4%、HPⅡ型21.9%,阴性31.2%; ②癌前病变组IL-10-1082 AG+GG基因型携带频率高于正常对照组(P=0.018), 携带IL-10-1082AG+GG基因型个体癌前病变的发病风险增高到1.801倍;③胃癌组IL-10-1082AG+GG基因型携带频率显著高于正常对照组(P= 0.010), 携带IL-10-1082AG+GG 基因型个体胃癌的发病风险增高到2.244倍;④当同时暴露于HP感染和基因突变两个因素时,研究对象罹患癌前病变、胃癌的风险分别为4.76倍、7.55倍;⑤健康对照组与癌前病变组相比交互作用指数S=2.53, 交 互 作 用 归 因 比AP=0.478.说明HP感染和IL-10基因多态性在罹患癌前病变时存在相加模型交互作用。⑥健康对照组与胃癌组相比交互作用指数S=3.43, 交互作 用归因AP=0.615.说明HP感染和IL-10基因多态性在罹患胃癌时存在相加模型交互作用。⑦吸烟、携带IL-10-1082AG+GG基因型者发生胃癌的风险增高OR=2.942倍(P=0.016)。 结论: IL-10基因-1082位点A/G多态性与胃癌的遗传易感性相关; Ⅰ型HP 感染会增加感染者罹患胃癌及癌前病变的风险;IL-10基因-1082位点基因多态性和 Hp感染在胃癌发生发展过程存在加乘交互作用。
Other Abstract【OBJECTIVE】 To investigate the relationship of Helicobacter pylori Infection, IL-10 polymorphism and susceptibility to gastric cancer in high incidence area of Hexi area of Gansu province. 【METHODS】 All investigated objects were divided into three groups: 151 gastric cancer patients, 148 precancerous lesion patients and 153 control subjects were enrolled. Patient clinical data were collected, including age, gender, epidemiological data, family history of gastric cancer, endoscopy or after the diagnosis of pathology results.The Interleukin-10-1082 promotr polymorphism was detected by SNaPshot; and Immunoblotting was performed to test H.pylori. The results were analyzed by SPSS15.0 【RESULTS】:①The distribution frequency of AA ,AG/GG two genotypes were75.8% ,24.2% respectively in normal control. ②The frequency of AG+GG genotype was statistically higher in the gastric precancerous lesion (GPL) group compared to the control group (P=0.018), moreover, individuals with the IL-10AG+GG genotype increased 1.801 fold risk for gastric precancerous lesion. ③The frequency of AG+GG genotype was conspicuous higher in the gastric cancer (GC) group compared to the control group (P=0.010), moreover, individuals with the IL-10AG+GG genotype increased 2.244 fold risk for gastric cancer. ④Participations exposed in both enviromental factor (H. pylori infection) and gene mutation factor (carrying IL-10-1082 AG/GG genotypes), the risk of developing gastric precancerous lesion and gastric cancer was 4.767,7.55 fold of those exposed in neither enviromental factor nor gene mutation factor(ORge,4.767,7.55). ⑤Healthy control group compared with the precancerous lesion group synergy index S = 2.53, attributable proportion of interaction AP = 0.478.⑥Healthy control group compared with the gastric cancer group, synergy index S = 3.432, attributable proportion of interaction AP = 0.615.⑦Smoking, carrying IL-10AG + GG genotype increased the risk of gastric cancer happen OR = 2.942 (P = 0.016); 【CONCLUSION】: Results indicated that there was a relationship between IL-10-1082A/G polymorphism and susceptibility to gastric cancer. And there was statistically interaction between the IL-10-1082 site A/G polymorphism in IL-10 and H. pylori infection in the occurrence and development process of gastric cancer in Hexi area of Gansu Province,China.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224081
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
曾祥挺. IL-10基因多态性与环境因素在甘肃河西地区胃癌高发中交互作用的研究[D]. 兰州. 兰州大学,2010.
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