|Other Abstract||Background and Objective Observation of insulin-like growth factor 1 ( insulin-like growth factor 1 IGF-1 ), CD34 ( marker of tumor vascular endothelial cells, to reflect microvessel density ) protein expression in normal colorectal mucosa, adenoma, colorectal carcinoma, And to explore the relationship between the expression and clinicopathological parameters in colorectal cancer IGF-1, CD34, to further explore the relationship with tumor angiogenesis.
Methods The expression of IGF-1 and CD34 was detected, Using immunohistochemical method ( two EnVision System ), in 20 cases of nomal tissue,54 cases of colorectal adenoma,56 cases of colorectal carcinoma. All cases are Proved by pathology. The vascular endothelial cells were marked by CD34, which it counts microvessel ( microvessel density MVD ).
Results 1, IGF-1 protein was weakly expressed in normal colorectal mucosa. IGF-1 protein expression was moderate in colorectal adenoma tissues, and mainly expressed in the cytoplasm. The IGF-1 protein expression was higher intensity in colorectal adenocarcinoma, and expressed mainly in the cytoplasm. The positive expression rate of IGF-1 in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma were 35%, 64.81%, 85.71%. Comparison of the positive rate of 3 kinds of tissues, adenoma was higher than that of normal mucosa, adenocarcinoma is higher than that of adenoma, adenocarcinoma was significantly higher than that in normal mucosa. They differences were statistically significant (P < 0.05). The expression of IGF-1 protein in patients with gender, age, size, site, DUCKE ′ S staging, there is no thrombi showed no significant correlation (P>0.05). The expression of IGF-1 protein in patients with degree of differentiation, depth of tumor invasion, lymph node metastasis were significantly correlated (P < 0.05). 2, CD34 protein expression was low in normal colon tissue, the MVD value was 8.7665 ± 2.66732; The CD34 protein expression was moderate in colorectal adenoma tissues, the MVD value was 22.5320 ± 5.69328; The CD34 protein expression was higher in the colorectal carcinoma, the MVD value was 35.5534 ± 7.77696. Adenoma group MVD value was significantly higher than that in normal colorectal mucosa, colorectal cancer tissues was significantly higher than that adenoma in group MVD, colorectal cancer tissues was significantly higher than that in normal mucosa group MVD, the groups was statistically significant (P<0.05). CD34 marker MVD value...|