兰州大学机构库 >学院待认领
HMGB1、HE4和CA125在上皮性卵巢癌患者血清中的表达情况及临床意义
Alternative TitleExpression and clinical significancen of HMGB1、HE4 and CA125 in epithelial ovarian carcinoma
贺小燕
Thesis Advisor王海琳
2011-05-16
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword上皮性卵巢癌 人高迁移率族蛋白(HMGB1) 人附睾蛋白(HE4) 癌抗原(CA125) 肿瘤标志物 酶联免疫吸附法(ELISA)
Abstract目的 探讨高迁移率族蛋白B1(high mobility group box-1,HMGB1)、人附睾蛋白(human epididymis protein,HE4)和CA125与上皮性卵巢癌的关系,并分析其在上皮性卵巢癌患者血清中的表达情况以及临床意义。 方法 采用酶联免疫吸附(Enzyme.1inked Immunosorbent Assay,ELISA))双抗体夹心法检测96例女性血清中HMGB1、HE4和CA125表达水平,其中66例盆腔肿物住院患者来自甘肃省人民医院妇产科,按照手术后病理结果分为卵巢良性肿瘤组40例和卵巢恶性肿瘤组26例;另外来自甘肃省人民医院体检中心30例健康女性作为正常对照组。分析其与上皮性卵巢癌发生、发展、浸润和转移的关系,以及与上皮性卵巢癌的不同分期和临床病理特征之间的关系;初步探讨作为肿瘤标志物在上皮性卵巢癌患者血清中检测的临床意义。 结果 1.上皮性卵巢癌组血清中HMGB1、HE4和CA125表达水平明显高于其他两组,比较差异有统计学意义(P<0.05);三者在卵巢良性肿瘤组患者血清中表达水平均高于正常对照组,但比较差异无统计学意义(P>0.05); 2. 上皮性卵巢癌有淋巴结及远处转移者血清中HMGB1表达水平明显高于无转移者,二者比较差异有统计学意义(P<0.05);FIGO为III—IV期患者HMGB1水平高于I—Ⅱ期,二者比较差异有统计学意义(P<0.05);病理类型不同的患者HMGB1表达不同,但是三组比较差异无统计学意义(P>0.05); 3. HE4在上皮性卵巢癌的不同分期和病理类型血清中表达水平不同,但比较均无统计学意义(P>0.05)。 4.以卵巢良性肿瘤组+健康对照组作参照,通过ROC曲线确定HMGB1和HE4的界点值(ROC曲线最左上方的点为敏感性和特异性之和最大的点,此点的测定值即为最佳临界值),得出HMGB1、H4的界点值分别为153.25ug/ml、74.68pmol/l,CA125的界点值依据临床使用定为35u/ml。在上皮性卵巢癌中检测HMGB1、HE4和CA125,其敏感度分别为78.6%、69.2%、80.8%,特异度为60.2%、97.1%、77%。 结论 1.HMGB1、HE4和CA125在上皮性卵巢癌患者血清中的表达水平都高于卵巢良性肿瘤组和正常对照组,有助于良恶性卵巢肿瘤的鉴别。 2.HMGB1在卵巢癌各期和各病理类型中表达水平不同,因此推测其与上皮性卵巢癌发生、发展、浸润和转移有关。 3. 对于上皮性卵巢癌患者的检测,CA125敏感度最高,HE4的特异度最高,二者与HMGB1联合检测对于上皮性卵巢癌的筛查和初步诊断是较佳组合。
Other AbstractObjective To study the relationship of high mobility group protein B1 (high mobility group box-1, HMGB1), human epididymal protein (human epididymis protein, HE4) and CA125 in epithelial ovarian cancer, and to analyse the expression and clinical significance in epithelial ovarian cancer . Methods The expression of HMGB1, HE4 and CA125 in serum were detected in 96 women by Enzyme 1inked Immunosorbent Assay(ELISA)) double antibody sandwich method. 66 hospitalized patients with pelvic masses from Gansu Provincial People's Hospital of Obstetrics and Gynecology were divided into 40 patients with benign ovarian tumors and 26 patients with ovarian cancer in accordance with pathological findings after surgery. 30 healthy women from Gansu Provincial People's Hospital Medical Center were regarded as control group. To analyse the relationship of these results with development, invasion, metastasis and different stages, clinicopathological features in epithelial ovarian cancer. And to discuss the clinical significance of HMGB1, HE4 and CA125 as tumor markers in epithelial ovarian cancer. Results 1. The expression of HMGB1, HE4 and CA125 was significantly higher than the other two groups in serum of epithelial ovarian cancer patients. There was significant difference (P <0.05). The expression of HMGB1, HE4 and CA125 in serum of ovarian benign tumor group was higher than the normal control group, but no significant difference (P> 0.05) 2 .The expression of HMGB1 in epithelial ovarian cancer patients with lymph node and distant metastasis was higher than those without metastasis,There was significant difference (P <0.05); The expression in patients with FIGO III-IV was higher than that of patients with I-Ⅱ.There was significant difference (P <0.05)。The expression of HMGB1 in patients with different pathological types was different, but no significant difference (P> 0.05). 3. The expression of HE4 in epithelial ovarian cancer with different stages and pathological types was difference, but no significant difference (P> 0.05). 4. The sensitivity of HMGB1, HE4 and CA125 in epithelial ovarian cancer were 78.6%, 69.2%, 80.8%,meanwhile the specificity were 60.2%, 97.1%, 77%. Conclusion 1. The expression of HMGB1, HE4 and CA125 in epithelial ovarian cancer were higher than ovarian benign tumor group and control group, these factors may contribute to the identification of benign and malignant ovarian tumors. 2. The expression of HMGB1 was different in various stag...
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224096
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
贺小燕. HMGB1、HE4和CA125在上皮性卵巢癌患者血清中的表达情况及临床意义[D]. 兰州. 兰州大学,2011.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[贺小燕]'s Articles
Baidu academic
Similar articles in Baidu academic
[贺小燕]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[贺小燕]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.