| ERK5信号通路介导下的流体剪切力对成骨细胞增殖作用的实验研究 |
| Alternative Title | The influence of fluid shear stess on osteoblastic cells proliferation through ERK5 signaling pathway
|
| 盛晓贇 |
| Thesis Advisor | 夏亚一
|
| 2012-05-18
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 流体剪切力
细胞增殖
ERK5
CylinD1
CDK4
|
| Abstract | 【目的】本实验主要探讨流体剪切力与ERK5信号通路在成骨细胞增殖反应中的作用关系，为确立骨组织中的机械应力传导机制提供依据。
、【结论】正常生理环境中，ERK5信号通路存在且参与成骨细胞的正常增殖活动。12dyne/cm2的FSS刺激可以通过ERK5-CylinD1-CDK4信号传导通路起到调节成骨细胞增殖活动的作用，短时间（t≤60min）持续FSS刺激可通过ERK5-CylinD1-CDK4信号传导通路正性调节细胞周期，促进成骨细胞增殖；而长时间（t>60min）FSS刺激则会抑制ERK5的磷酸化，通过ERK5-CylinD1-CDK4信号传导通路调控细胞周期调控因子起到抑制细胞增殖的作用。 |
| Other Abstract | 【Object】This study explore to the involvement of ERK5 signaling pathway in the proliferation response of osteoblastic cells to fluid shear stress , to provide the basis to establish the transmission mechanism of mechanical stress in the bone tissue.
【Conclusion】Normal physiological environment，ERK5 signaling pathway is involved in the proliferative activity of osteoblasts. The 12dyne/cm2 FSS stimulus can through the ERK5-CylinD1-CDK4 signaling transduction pathway play a positive regulatory role of osteoblasts proliferation activity, the short time (t≤60min) continuous FSS stimulation of ERK5-CylinD1-CDK4 in signaling transduction pathway positive regulation of cell cycle and promote the proliferation of osteoblasts; the long time(>60min) FSS stimulation will inhibit phosphorylation of ERK5, then by ERK5-CylinD1-CDK4 signaling transduction pathway on cell cycle regulatory factors play a role in inhibiting cell proliferation.
Key words:Fluid shear stress; Cell proliferation; ERK5; CylinD1; CDK4 |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/224146
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
盛晓贇. ERK5信号通路介导下的流体剪切力对成骨细胞增殖作用的实验研究[D]. 兰州. 兰州大学,2012.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
