兰州大学机构库 >学院待认领
ARHGEF11基因R1467H多态性与甘肃回族、汉族2型糖尿病的相关性研究
Alternative TitleAssociation of ARHGEF11 gene R1467H polymorphism with type 2 diabetes mellitus in Gansu Hui and Han population
陈小慧
Thesis Advisor刘静
2010-06-01
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
KeywordRho蛋白鸟苷酸交换因子11基因 R1467H多态性 2型糖尿病 胰岛素抵抗
Abstract目的 明确ARHGEF11 (Rho guanine nucleotide exchange factor 11,Rho蛋白鸟苷酸交换因子11) 基因R1467H位点多态性在甘肃回、汉两民族人群中的分布及其差异性;初步探讨ARHGEF11基因R1467H位点多态性与甘肃回、汉族人群2型糖尿病、胰岛素抵抗、肥胖及血脂异常的相关性。 方法 1. 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和DNA直接测序技术,分别对回族117例2型糖尿病患者(T2DM组)、104例正常对照者(NC组)和汉族149例2型糖尿病患者(T2DM组)、114例正常对照者(NC组)ARHGEF11基因R1467H多态性位点进行基因分型,并分析回、汉族人群两组基因型和等位基因频率的差异。 2. 采用全自动生化分析仪测定空腹血糖(FPG)、血脂、肝功能、肾功能,糖化血红蛋白分析仪测定糖化血红蛋白(HbA1c),放射免疫法测定空腹胰岛素(FINS)。 3. 同时测量身高、体重、血压、腰围、臀围,计算体重指数(BMI)=体重/身高2(Kg/m2),腰臀比(WHR) =腰围/臀围,HOMA胰岛素抵抗指数(HOMA-IR) =空腹血糖(FPG)×空腹胰岛素(FINS)/22.5。 结论 ARHGEF11基因R1467H位点多态性在甘肃回族和汉族人群中的分布不存在差异;ARHGEF11基因R1467H位点多态性可能与甘肃汉族人群胰岛素抵抗及2型糖尿病相关,亦可能与甘肃回族人群2型糖尿病、肥胖及血脂异常相关。
Other AbstractObjectives The aim of our study was to investigate the distribution and difference of ARHGEF11 gene R1467H polymorphism in Hui and Han population in Gansu province. To evaluate the relationship between the R1467H polymorphism in ARHGEF11 and risk of type 2 diabetes mellitus (T2DM) and insulin resistance in Hui and Han population in Gansu province. Methods We genotyped R1467H polymorphism in 117 patients with T2DM and 104 control subjects in Gansu Hui population and 149 patients with T2DM and 114 control subjects in Gansu Han population, using a polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) protocol and DNA sequencing methods. The frequencies of genotypes and alleles at the site were compared between the T2DM and control groups. Total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and fasting blood glucose levels were determined by automatic biochemistry analyzer. Glycosylated hemoglobin (HbA1c) value was determined by HbA1c analyzer. Fasting insulin concentration was measured using insulin radioimmunoassay kit. At the same time, we measured the height, weight, waist circumference, hip circumference, blood pressure. The body mass index (BMI), waist hip ratio (WHR) and insulin resistance index (HOMA-IR) were computed. Conclusion There was no significant difference in distribution of ARHGEF11 R1467H polymorphism between T2DM and NC in Hui and Han population of Gansu province. Our investigation suggests that the R1467H polymorphism of ARHGEF11 gene may contribute to susceptibility to T2DM and insulin resistance in Han population in Gansu province. Moreover, the SNP was likely related to increased risk of T2DM, obesity and dyslipidemia in Hui population in Gansu province.
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/224224
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
陈小慧. ARHGEF11基因R1467H多态性与甘肃回族、汉族2型糖尿病的相关性研究[D]. 兰州. 兰州大学,2010.
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