AG014699联合顺铂抑制人卵巢癌SKOV3细胞增殖及诱导其凋亡的研究

兰州大学机构库 >学院待认领

	AG014699联合顺铂抑制人卵巢癌SKOV3细胞增殖及诱导其凋亡的研究
Alternative Title	Effects of AGO14699 alone and combined with cisplatin on Proliferation and Cellular Apoptosis of Human Ovarian Cancer Cell Line SKOV3
	摆扬
Thesis Advisor	王海琳
	2012-05-17
Degree Grantor	兰州大学
Place of Conferral	兰州
Degree Name	硕士
Keyword	卵巢癌 SKOV3 CDDP AG014699
Abstract	目的研究选择性PARP-1抑制剂AG014699联合CDDP对人卵巢癌SKOV3细胞增殖、周期及凋亡的影响，为临床应用其作为治疗卵巢癌的新辅助抗癌药物提供实验和理论依据。方法 1.体外培养人卵巢癌SKOV3细胞，不同浓度的AG014699、CDDP、AG014699+CDDP处理，倒置显微镜及MTT法观察各组细胞增殖抑制情况;2.FCM分析细胞周期改变及其对细胞凋亡的影响。结果 1. 倒置显微镜见对照组细胞均匀贴壁生长，相邻细胞融合成片；单药AG014699、CDDP组细胞生长受限明显；联合用药组细胞从瓶壁脱落，脱落细胞悬浮连接成片。2. MTT法示不同浓度的AG014699和CDDP单独和联合用药均可抑制SKOV3细胞的生长，并且具有较明显的剂量依赖关系。3.流式细胞术示AG014699阻滞细胞于G0/G1期，联合用药显著阻滞细胞于G0/G1期且细胞凋亡率明显上升。结论 AG014699可抑制人卵巢癌细胞株SKOV3细胞体外增殖及凋亡、阻滞细胞于G0/G1期、联合用药可增强CDDP的疗效。关键词卵巢癌，SKOV3，AG014699，CDDP
Other Abstract	Objective: To investigate effects of AG014699 combined with CDDP on proliferation,cell cycle,apoptosis of SKOV3. To provide evidence of AG014699 as the combination with chemotherapy on ovarian cancer. Methods: SKOV3 were treated with AG014699,CDDP,AG014699+CDDP.Inverted microscope was used to detect apoptosis. Growth inhibition effect was investigated by MTT , distribution of cell cycles and apoptosis ratio were analyzed by FCM. Results: 1.Under the microscope,control group cells growth confluent; Single drug AG014699 and CDDP groups seemed bad; The combined group even bad.2.MTT showed AG014699 alone and combined with CDDP exerted inhibitory effect on proliferation.3.FCM showed AG014699 could block cell cycle in G0/G1 . Combined with CDDP, AG014699 could reinforce its effect and apoptosis ratio were enhanced. Conclusion: AG014699 could inhibit proliferation of SKOV3,block cell cycle,induce cell apoptosis.Combined with CDDP, the effects were reinforced. Key words: Ovarian cancer; SKOV3; AG014699; Cisplatin
URL	查看原文
Language	中文
Document Type	学位论文
Identifier	https://ir.lzu.edu.cn/handle/262010/224243
Collection	学院待认领
Affiliation	临床医学院
Recommended Citation GB/T 7714	摆扬. AG014699联合顺铂抑制人卵巢癌SKOV3细胞增殖及诱导其凋亡的研究[D]. 兰州. 兰州大学,2012.

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