| 2013-2014年兰州地区婴幼儿病毒性腹泻的流行病学研究 |
| Alternative Title | Epidemiologic Study for Viral Infantile Diarrhea in Lanzhou Area in 2013-2014
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| 谢婧 |
| Thesis Advisor | 李宇宁
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| 2015-05-17
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 病毒性腹泻
婴幼儿
轮状病毒
杯状病毒
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| Abstract | 目的：通过了解兰州地区2013-2014年度轮状病毒、杯状病毒、腺病毒、星状病毒等引起婴幼儿病毒性腹泻常见病 原体的流行病 学特点，对病毒性腹泻的预防和控制提供数据支持和理论依据。
方法：采集2013年7月至2014年6月兰州大学第一医院5岁以下腹泻婴幼儿的232份粪便标本，同时个案表收集其个人资料及临床表现情况。采用酶联免疫吸附试验（ELISA）及巢氏逆转录PCR法检测A组轮状病毒（HRVA）阳性标本并进一步G/P分型；逆转录PCR检测诺如病毒（NoV）；PCR检测腺病毒（HAdV）；实时定量逆转录PCR检测札如病毒（SapV）及星状病毒（HAstV）。
结果：在待测232份标本中，有146份标本检出上述病毒感染。其中轮状病毒检出率为40.09%，其次是杯状病毒，腺病毒和星状病毒。
结论：轮状病毒为兰州地区婴幼儿腹泻主要病原体；本年度诺如病毒流行株GII.4与GII.4/Sydney2012变异株同源。 |
| Other Abstract | Objective：To analyze the features of epidemiology and clinical characteristics of Group A Rotavirus (GARV), Human Calicivirus (HuCV), Human Adenovirus (HAdV) and Human Astrovirus (HAstV) in Lanzhou area of China from 2013 to 2014, and to provide data support and theoretical basis for the prevention and control of viral diarrhea.
Methods：A total of 232 stool samples were collected from hospitalized children less than five years with acute infection diarrhea, in Department of Pediatrics, the First Hospital of Lanzhou University, between July 2013 and June 2014. At same time, personal information and clinical data of those children were collected. Group A rotavirus was identified by enzyme-linked immunosorbent assay (ELISA), while G and P serotypes were identified by nested reverse transcription PCR method. Norovirus was confirmed by reverse transcription PCR method and Adenovirus by PCR method. Sapovirus and Astrovirus were detected by real-time quantitative reverse transcription PCR method.
Results：Among the 232 samples, 146 samples were detected with virus infection, with a rate of 62.93%, indicating that the virus infection was the primary cause for diarrhea among the infants in Lanzhou area this year. The infection rate of Rotavirus was 40.09% (93/232), which was the most prevalent one among the four viruses, then followed by Calicivirus （41/232，17.67%）. The detection rate of Adenovirus and Astrovirus were 7.33% and 6.03% respectively. There were 18 co-infections(7.76%) to be found while most of which were Rotavirus mixing with other viruses.
Conclusion：Rotavirus was the main pathogen for diarrhea in infants in Lanzhou area. The main epidemic strain GII.4 of Norovirus this year showed homology with the variant strain GII.4/Sydney2012 reported in Sydney in 2012. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/224290
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
谢婧. 2013-2014年兰州地区婴幼儿病毒性腹泻的流行病学研究[D]. 兰州. 兰州大学,2015.
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