|Alternative Title||Mechanical Property Measurement of Cancer Cells and Biomechanics of Cell Damage Induced by Irradiation
|Place of Conferral||兰州
|Other Abstract||Based on the induction effect of cell apoptosis by heavy ion RT, this study established a canonical cell apoptosis model in vitro and cell damage model in vivo. By means of molecular biology methods, such as MTT assay, and western blotting technique and combining with single cell mechanics technique based on atomic force microscopy (AFM) and clinical cytology analysis, we investigate how the change of cell mechanical properties would be brought into to the research system to assess cancer killing mechanism, therapeutic effect and adverse effects of radiation.
Obtained innovative research results as follows:
(1) Based on the change of mechanical properties to characterize the differences between cancer cells and normal cells, we found that hepatocellular carcinoma cell (HCC) lines showed the smaller value of cell elasticity compared with normal hepatocyte (NH) line (more softer), with the increasing of the HCC invasiveness, which exhibited a lower cell elasticity value. Meawhile, cell adhesion force and surface adhesion energy increased with the enhancing of invasive potential in the HCCs.
(2) Based on the cancer cell-killing effects by carbon ion irradiation, five HCCs and one NHs were treated with CII technique to establish a canonical cell apoptosis model by regualting the molecular pathways in vitro. From the perspective of the mechanical signatures to characterize, we found a close relationship during the apoptotic process that appears to exist among the cell microstructure, cell mechanical properties, actin cytoskeletal organization, and expression of apoptotic signaling molecules. These findings imply that the cell mechanical properties, morphological remodeling and molecular pathways are tightly coupled interaction to regulate the progression of apoptosis. Furthermore, based on the classical morphological features during the the onset of apoptosis, for the first time, we proposed a theoretical model of apoptosis which revealed that the characteristic change in cellular budding of apoptotic bodies was mainly dominated by the mechanical mechanism.
(3) Based on the viewpoint of irradiation-induced damage-effect evaluation of erythrocytes in the Syrian hamsters, this clarify the biomechanical mechanism of ionizing radiation induction to erythrocytes damage from two aspects between mechanical properties and configuration changes. As well, we for the first time put forward the change of erythrocyte rigidity to evaluate the ionizing radiation damage ...|
张宝平. 癌细胞力学性质测量及放射诱导下细胞损伤的生物力学研究[D]. 兰州. 兰州大学,2016.
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