| 7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis | |
| Wu, Chengxin1; Sun, Yuanyuan1; Wang, Yuanhang1; Duan, Wenzhen2; Hu, Jiangyue1; Zhou, Lei1; Pu, Qiaosheng1 | |
| 2019-08-15 | |
| Source Publication | TALANTA Impact Factor & Quartile |
| ISSN | 0039-9140 |
| EISSN | 1873-3573 |
| Volume | 201Pages:16-22 |
| page numbers | 7 |
| Abstract | Diode lasers, especially 405 nm diode laser, as excitation sources offer new opportunities for laser-induced fluorescence (LIF) detection, but the lack of available derivatization reagents limits their widespread applications. Herein, a commercial fluorescent dye, 7-(diethylamino)coumarin-3-carboxylic acid (DEAC-C) was introduced as derivatization reagent and corresponding derivatization strategy for DEAC-C labeling sulfonamides was developed for 405 nm LIF detection. After systematic optimization, three sulfonamides including hydrochlorothiazide (HCTZ), chlorothiazide (CTZ) and chlortalidone (CTD) could be efficiently labeled by DEAC-C in the presence of cyanuric chloride and triethylamine (1.5%) in acetonitrile at 50 degrees C for 180 min. Based on the laboratory-built capillary electrophoresis-LIF system, a robust method was then proposed for the separation of DEAC-C labeled sulfonamides by micellar electrokinetic chromatography (MEKC) using the classic borax-SDS system. Under the optimized conditions, a baseline separation of three sulfonamides was achieved within 15 min, and the detection limits were determined to be 0.24, 0.29, and 0.23 nM for HCTZ, CTZ, and CTD, respectively. Furthermore, the developed MEKC-LIF method was validated using sulfonamide standards and spiked human urine sample and successfully applied for the analysis of three sulfonamides in complex pharmaceutical and physiological samples. |
| Keyword | 7-(Diethylamino)coumarin-3-carboxylic acid Derivatization Diuretic Laser-induced fluorescence Micellar electrokinetic chromatography Sulfonamide |
| Publisher | ELSEVIER SCIENCE BV |
| DOI | 10.1016/j.talanta.2019.03.093 |
| Indexed By | SCIE |
| Language | 英语 |
| Funding Project | Fundamental Research Funds for the Central Universities[lzujbky-2017-111] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000471084300003 |
| Publisher | ELSEVIER SCIENCE BV |
| Original Document Type | Review |
| PMID | 31122407 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/295360 |
| Collection | 兰州大学 |
| Corresponding Author | Zhou, Lei |
| Affiliation | 1.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Coll Earth & Environm Sci, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Chengxin,Sun, Yuanyuan,Wang, Yuanhang,et al. 7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis[J]. TALANTA,2019,201:16-22. |
| APA | Wu, Chengxin.,Sun, Yuanyuan.,Wang, Yuanhang.,Duan, Wenzhen.,Hu, Jiangyue.,...&Pu, Qiaosheng.(2019).7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis.TALANTA,201,16-22. |
| MLA | Wu, Chengxin,et al."7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis".TALANTA 201(2019):16-22. |
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