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Coexpression of PalbHLH1 and PalMYB90 Genes From Populus alba Enhances Pathogen Resistance in Poplar by Increasing the Flavonoid Content
Bai, Qiuxian; Duan, Bingbing; Ma, Jianchao; Fen, Yannan; Sun, Shujiao; Long, Qiming; Lv, Jiaojiao; Wan, Dongshi
2020-02-26
Source PublicationFrontiers in Plant Science   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN1664-462X
Volume10
page numbers14
AbstractSecondary metabolites of the flavonoid pathway participate in plant defense, and bHLH and MYB transcription factors regulate the synthesis of these metabolites. Here, we define the regulatory mechanisms in response to pathogens. Two transcription factors from Populus alba var. pyramidalis, PalbHLH1 and PalMYB90, were overexpressed together in poplar, and transcriptome analysis revealed differences in response to pathogen infection. The transgenic plants showed elevated levels of several key flavonoid pathway components: total phenols, proanthocyanidins (PAs), and anthocyanins and intermediates quercetin and kaempferol. Furthermore, PalbHLH1 and PalMYB90 overexpression in poplar enhanced antioxidase activities and H2O2 release and also increased resistance to Botrytis cinerea and Dothiorella gregaria infection. Gene expression profile analysis showed most genes involved in the flavonoid biosynthesis pathway or antioxidant response to be upregulated in MYB90/bHLH1-OE poplar, but significant differential expression occurred in response to pathogen infection. Specifically, expression of PalF3H (flavanone 3-hydroxylase), PalDFR (dihydroflavonol 4-seductase), PalANS (anthocyanin synthase), and PalANR (anthocyanin reductase), which function in initial, middle, and final steps of anthocyanin and PA biosynthesis, respectively, was significantly upregulated in D. gregaria-infected MYB90/bHLH1-OE poplar. Our results highlight that PalbHLH1 and PalMYB90 function as transcriptional activators of flavonoid pathway secondary-metabolite synthesis genes, with differential mechanisms in response to bacterial or fungal infection.
KeywordPalbHLH1 and PalMYB90 Populus alba var pyramidalis flavonoid Dothiorella gregaria Sacc Botrytis cinerea
PublisherFRONTIERS MEDIA SA
DOI10.3389/fpls.2019.01772
Indexed BySCIE
Language英语
Funding ProjectNational Science Foundation of China[31870580] ; Fundamental Research Funds for the Central Universities[lzujbky-2017-k14]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000526547200001
PublisherFRONTIERS MEDIA SA
Original Document TypeArticle
PMID 32174927
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/368853
Collection生命科学学院
Corresponding AuthorWan, Dongshi
Affiliation
Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou, Peoples R China
First Author AffilicationSchool of Life Sciences
Corresponding Author AffilicationSchool of Life Sciences
First Signature AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Bai, Qiuxian,Duan, Bingbing,Ma, Jianchao,et al. Coexpression of PalbHLH1 and PalMYB90 Genes From Populus alba Enhances Pathogen Resistance in Poplar by Increasing the Flavonoid Content[J]. Frontiers in Plant Science,2020,10.
APA Bai, Qiuxian.,Duan, Bingbing.,Ma, Jianchao.,Fen, Yannan.,Sun, Shujiao.,...&Wan, Dongshi.(2020).Coexpression of PalbHLH1 and PalMYB90 Genes From Populus alba Enhances Pathogen Resistance in Poplar by Increasing the Flavonoid Content.Frontiers in Plant Science,10.
MLA Bai, Qiuxian,et al."Coexpression of PalbHLH1 and PalMYB90 Genes From Populus alba Enhances Pathogen Resistance in Poplar by Increasing the Flavonoid Content".Frontiers in Plant Science 10(2020).
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