兰州大学机构库 >草地农业科技学院
绵羊ZFY基因单核苷酸多态性标记的检测方法及标记应用
乐祥鹏
Creator乐祥鹏 ; 曹学涛 ; 秦芳 ; 李发弟 ; 李万宏
2019-05-10
Rights Holder兰州大学
Country中国
Subtype发明申请
Abstract本发明公开了绵羊ZFY基因的单核苷酸多态性标记的检测方法及标记应用。该标记是绵羊ZFY基因片段第403位的G或A的单核苷酸多态性。检测引物包括正向引物和反向引物,分别为如SEQ ID NO.1和SEQ ID NO.2所示的核苷酸序列。检测方法包括:提取待测公绵羊全基因组DNA;以待测公绵羊全基因组DNA为模板,以母羊DNA为阴性对照,以水为空白对照,利用检测引物进行 PCR 扩增绵羊ZFY基因;用限制性内切酶Bsp1286                                               消化PCR扩增产物之后,再对酶切后的扩增片段进行琼脂糖凝胶电泳;根据电泳结果鉴定绵羊ZFY基因上单核苷酸多态性位点的基因型。
Application Date2019-01-05
Patent NumberCN201910009752.7
Status实质审查
Application NumberCN201910009752.7
Open (Notice) NumberCN109735630A
IPC Classification NumberC12Q1/6888 | C12Q1/6879 | C12Q1/683
Patent Agent陈千
Agency成都时誉知识产权代理事务所(普通合伙)
Document Type专利
Identifierhttps://ir.lzu.edu.cn/handle/262010/413278
Collection草地农业科技学院
Affiliation
兰州大学
First Author AffilicationLanzhou University
Recommended Citation
GB/T 7714
乐祥鹏. 绵羊ZFY基因单核苷酸多态性标记的检测方法及标记应用. CN201910009752.7[P]. 2019-05-10.
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