兰州大学机构库 >草地农业科技学院
绵羊EIF2S3Y基因的单核苷酸多态性标记及其检测引物、试剂盒、方法及应用
乐祥鹏
Creator乐祥鹏 ; 曹学涛 ; 李发弟 ; 李万宏 ; 秦芳
2018-12-14
Rights Holder兰州大学
Country中国
Subtype发明申请
Abstract本发明提供一种绵羊EIF2S3Y基因的单核苷酸多态性标记及其检测引物、试剂盒、方法及应用。该标记是绵羊EIF2S3Y基因片段第61位的G或C的单核苷酸多态性。检测引物包括正向引物和反向引物,分别为如SEQ ID NO.1和SEQ ID NO.2所示的核苷酸序列。检测方法包括:提取待测公绵羊全基因组DNA;以待测公绵羊全基因组DNA为模板,以母羊DNA为阴性对照,以水为空白对照,利用检测引物进行PCR扩增绵羊EIF2S3Y基因;用限制性内切酶AciI消化PCR扩增产物之后,再对酶切后的扩增片段进行琼脂糖凝胶电泳;根据电泳结果鉴定绵羊EIF2S3Y基因上单核苷酸多态性位点的基因型。
Application Date2018-08-18
Patent NumberCN201810943879.1
Status实质审查
Application NumberCN201810943879.1
Open (Notice) NumberCN108998540A
IPC Classification NumberC12Q1/6888 | C12N15/11
Patent Agent韩晓银
Agency成都天汇致远知识产权代理事务所(普通合伙)
Document Type专利
Identifierhttps://ir.lzu.edu.cn/handle/262010/413604
Collection草地农业科技学院
Affiliation
兰州大学
First Author AffilicationLanzhou University
Recommended Citation
GB/T 7714
乐祥鹏. 绵羊EIF2S3Y基因的单核苷酸多态性标记及其检测引物、试剂盒、方法及应用. CN201810943879.1[P]. 2018-12-14.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[乐祥鹏]'s Articles
Baidu academic
Similar articles in Baidu academic
[乐祥鹏]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[乐祥鹏]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.