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The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens
Ali, Muhammad Amjad1,2,3; Abbas, Amjad1,3; Azeem, Farrukh4; Shahzadi, Mahpara1,5; Bohlmann, Holger3
2020-03
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
EISSN1422-0067
Volume21Issue:5
page numbers21
AbstractArabidopsis contains 34 genes for glycosylphosphatidylinositol (GPI)-anchored LTPg proteins. A motif analysis has placed these into four groups. With one exception, all are produced with a signal peptide and are most likely attached to the cell membrane via the GPI anchor. Several of the LTPg genes across the four groups are downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii. We have here studied At3g22600 encoding LTPg5, which is the most strongly downregulated LTPg gene. It is mainly expressed in roots, and a promoter::GUS line was used to confirm the downregulation in syncytia and also showed downregulation in galls of the root knot nematode Meloidogyne incognita. In contrast, infection with bacteria (Pseudomonas syringae) and fungi (Botrytis cinerea) led to the induction of the gene in leaves. This diverse regulation of LTPg5 indicated a role in resistance, which we confirmed with overexpression lines and a T-DNA mutant. The overexpression lines were more resistant to both nematode species and to P. syringae and B. cinerea, while a knock-out mutant was more susceptible to H. schachtii and P. syringae. Thus, LTPg5 encoded by At3g22600 is part of the Arabidopsis resistance mechanism against pathogens. LTPg5 has probably no direct antimicrobial activity but could perhaps act by associating with a receptor-like kinase, leading to the induction of defense genes such as PR1.
Keywordlipid transfer protein antimicrobial peptide Arabidopsis Heterodera schachtii plant defense
PublisherMDPI
DOI10.3390/ijms21051774
Indexed BySCIE
Language英语
Funding ProjectAustrian Science Fund (FWF)[P20471-B11][P27217-B22] ; Ernst Mach-Follow-up grant from OeAD-GmbH, Vienna[ICM-2017-09646]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000524908500225
PublisherMDPI
Original Document TypeArticle
PMID 32150834
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/416812
Collection兰州大学
草地农业科技学院
Corresponding AuthorAli, Muhammad Amjad; Bohlmann, Holger
Affiliation
1.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad 38040, Pakistan
2.Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad 38040, Pakistan
3.Univ Nat Resources & Life Sci, Dept Crop Sci, Inst Plant Protect, A-1180 Vienna, Austria
4.GC Univ, Dept Bioinformat & Biotechnol, Plant Biotechnol Lab, Faisalabad 38040, Pakistan
5.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Grassland Econ & Syst Anal Lab, Lanzhou 730000, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Ali, Muhammad Amjad,Abbas, Amjad,Azeem, Farrukh,et al. The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(5).
APA Ali, Muhammad Amjad,Abbas, Amjad,Azeem, Farrukh,Shahzadi, Mahpara,&Bohlmann, Holger.(2020).The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(5).
MLA Ali, Muhammad Amjad,et al."The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.5(2020).
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