兰州大学机构库
lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs
2019-12-31
Source PublicationAging-US   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN1945-4589
Volume11Issue:24Pages:12476-12496
page numbers21
AbstractUnderstanding the bone and musculoskeletal system is essential to maintain the health and quality of life of our aging society. Mesenchymal stem cells (MSCs) can undergo self-renewal and differentiate into multiple tissue types including bone. We demonstrated that BMP9 is the most potent osteogenic factors although molecular mechanism underlying BMP9 action is not fully understood. Long noncoding RNAs (lncRNAs) play important regulatory roles in many physiological and/or pathologic processes. Here, we investigated the role of lncRNA Rmst in BMP9-induced osteogenic differentiation of MSCs. We found that Rmst was induced by BMP9 through Smad signaling in MSCs. Rmst knockdown diminished BMP9-induced osteogenic, chondrogenic and adipogenic differentiation in vitro, and attenuated BMP9-induced ectopic bone formation. Silencing Rmst decreased the expression of Notch receptors and ligands. Bioinformatic analysis predicted Rmst could directly bind to eight Notch-targeting miRNAs, six of which were downregulated by BMP9. Silencing Rmst restored the expression of four microRNAs (miRNAs). Furthermore, an activating Notch mutant NICD1 effectively rescued the decreased ALP activity caused by Rmst silencing. Collectively, our results strongly suggest that the Rmst-miRNA-Notch regulatory axis may play an important role in mediating BMP9-induced osteogenic differentiation of MSCs.
Keywordmesenchymal stem cells BMP9 long noncoding RNAs IncRNA Rmst miRNAs
PublisherIMPACT JOURNALS LLC
DOI10.18632/aging.102583
Indexed BySCIE
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFC1000803] ; National Institutes of Health[CA226303] ; U.S. Department of Defense[OR130096] ; Medical Scientist Training Program of the National Institutes of Health[T32 GM007281] ; University of Chicago Cancer Center Support Grant[P30CA014599] ; National Center for Advancing Translational Sciences of the National Institutes of Health[UL1 TR000430]
WOS Research AreaCell Biology ; Geriatrics & Gerontology
WOS SubjectCell Biology ; Geriatrics & Gerontology
WOS IDWOS:000505153600044
PublisherIMPACT JOURNALS LLC
Original Document TypeArticle
PMID 31825894
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/417680
Collection兰州大学
第一临床医学院
第二临床医学院
Corresponding AuthorShao, Zengwu; He, Tong-Chuan
Affiliation
1.Huazhong Univ Sci & Technol, Union Hosp, Dept Orthopaed, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China
2.Univ Chicago, Med Ctr, Mol Oncol Lab, Dept Orthopaed Surg & Rehabil Med, Chicago, IL 60637 USA
3.Chongqing Med Univ, Minist Educ, Key Lab Diagnost Med, Chongqing 400016, Peoples R China
4.Chongqing Med Univ, Sch Lab Med, Chongqing 400016, Peoples R China
5.Chongqing Med Univ, Affiliated Hosp, Chongqing 400016, Peoples R China
6.Lanzhou Univ, Key Lab Orthopaed Surg Gansu Prov, Hosp 1, Lanzhou 730030, Gansu, Peoples R China
7.Lanzhou Univ, Dept Orthopaed Surg, Hosp 1, Lanzhou 730030, Gansu, Peoples R China
8.Lanzhou Univ, Dept Obstet & Gynecol, Hosp 1, Lanzhou 730030, Gansu, Peoples R China
9.Lanzhou Univ, Key Lab Orthopaed Surg Gansu Prov, Hosp 2, Lanzhou 730030, Gansu, Peoples R China
10.Lanzhou Univ, Dept Orthopaed Surg, Hosp 2, Lanzhou 730030, Gansu, Peoples R China
11.Lanzhou Univ, Dept Obstet & Gynecol, Hosp 2, Lanzhou 730030, Gansu, Peoples R China
12.Soochow Univ, Dept Gen Surg, Affiliated Hosp 2, Suzhou 215004, Peoples R China
13.Chongqing Gen Hosp, Dept Orthopaed Surg, Chongqing 400013, Peoples R China
14.Chongqing Gen Hosp, Dept Lab Med, Chongqing 400013, Peoples R China
15.Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China
16.Sichuan Univ, Dept Burn & Plast Surg, West China Hosp, Chengdu 610041, Sichuan, Peoples R China
17.Guangzhou Univ Chinese Med, Affiliated Hosp 1, Dept Orthopaed Surg, Guangzhou 510405, Guangdong, Peoples R China
18.Wuhan Univ, Dept Orthopaed Surg, Affiliated Zhongnan Hosp, Wuhan 430072, Hubei, Peoples R China
19.Nanjing Med Univ, Dept Orthopaed Surg, BenQ Med Ctr, Nanjing 210000, Jiangsu, Peoples R China
20.Qingdao Univ, Dept Orthopaed Surg, Affiliated Hosp, Qingdao 266061, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhicai,Liu, Jianxiang,Zeng, Zongyue,et al. lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs[J]. Aging-US,2019,11(24):12476-12496.
APA Zhang, Zhicai.,Liu, Jianxiang.,Zeng, Zongyue.,Fan, Jiaming.,Huang, Shifeng.,...&He, Tong-Chuan.(2019).lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs.Aging-US,11(24),12476-12496.
MLA Zhang, Zhicai,et al."lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs".Aging-US 11.24(2019):12476-12496.
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