兰州大学机构库
Chronic CagA-positive Helicobacter pylori infection with MNNG stimulation synergistically induces mesenchymal and cancer stem cell-like properties in gastric mucosal epithelial cells
Lin, L(林利)1,2; Wei, HL(魏虎来)1; Yi, J(易娟)1; Xie, B(谢蓓)1; Chen, J(陈静)1; Zhou, Cunmin1; Wang, L(王莉)1; Yang, Yue1
2019-10
Source PublicationJOURNAL OF CELLULAR BIOCHEMISTRY   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN0730-2312
EISSN1097-4644
Volume120Issue:10Pages:17635-17649
page numbers15
AbstractA CagA-positive Helicobacter pylori (H. pylori) infection can cause malignant transformation of human gastric mucosal epithelial cells, and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is a chemical carcinogen that induces gastric carcinogenesis. Whether this environmental chemocarcinogen may synergistically enhance the risk of H. pylori-infected gastric cancer remains unclear. In this study, we adopted a chronic CagA-positive H. pylori infection with or without MNNG coinduction to establish a cellular model in GES-1 cells and an animal model in C57BL/6J mice. The proliferation, cell phenotype, apoptosis, epithelial-mesenchymal transition (EMT), stemness and tumorigenicity of gastric mucosal epithelial cells were analyzed in vitro and in vivo. The results showed that chronic H. pylori-infected GES-1 cells displayed inhibited apoptosis, abnormal proliferation, enhanced invasion, and migration, increased EMT/mesenchymal phenotype, colony formation and stem cell-like properties, and enhanced tumorsphere-formatting efficiency as well as CD44 expression, a known gastric cancer stem cell (CSC) marker. MNNG synergistically promoted the above actions of chronic H. pylori infection. Further studies in chronic H. pylori-infected C57BL/6J mice models showed that an increased incidence of premalignant lesions in the gastric mucosa tissue of the H. pylori-infected mice had occurred, the mouse gastric mucosa cells exhibited similar mesenchymal and CSC-like properties in the above GES-1 cells, and precancerous lesions and EMT/CSC-like phenotypes were reinforced by the synergistic action of MNNG stimulation. H. pylori infection and/or MNNG induction were capable of causing enhanced expression and activation of Wnt2 and beta-catenin, indicating that the Wnt/beta-catenin pathway is involved in the actions of H. pylori and MNNG. Taken together, these findings suggest that chronic CagA-positive H. pylori infection with MNNG stimulation synergistically induces mesenchymal and CSC-like properties of gastric mucosal epithelial cells.
KeywordC57BL 6 mice CagA-positive Helicobacter pylori cancer stem cell-like properties epithelial-mesenchymal transition (EMT) Immortalized human gastric epithelial cells (GES-1) N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) Wnt beta-catenin signaling pathway
PublisherWILEY
DOI10.1002/jcb.29031
Indexed BySCIE
Language英语
Funding ProjectFundamental Research Funds for the Central Universities from Lanzhou University, Gansu Province, China[lzujbky-2017-142] ; Project of Scientific and technical guidance of Lanzhou, Gansu Province, China[2017-ZD-31] ; Science and Technology Planning Project from Chengguan District, Lanzhou, Gansu Province, China[2016-7-11] ; Young Talents Innovation Projects from Gansu Provincial Cancer Hospital, Gansu Provincial Academic Institute for Medical Research, China[ykz-2018-1]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000483551100121
PublisherWILEY
Original Document TypeArticle
PMID 31209915
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/418224
Collection兰州大学
基础医学院
Corresponding AuthorWei, Hulai
Affiliation
1.Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China
2.Gansu Prov Canc Hosp, Hematol Dept, Lanzhou, Gansu, Peoples R China
First Author AffilicationSchool of Basic Medical Sciences
Corresponding Author AffilicationSchool of Basic Medical Sciences
First Signature AffilicationSchool of Basic Medical Sciences
Recommended Citation
GB/T 7714
Lin, Li,Wei, Hulai,Yi, Juan,et al. Chronic CagA-positive Helicobacter pylori infection with MNNG stimulation synergistically induces mesenchymal and cancer stem cell-like properties in gastric mucosal epithelial cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2019,120(10):17635-17649.
APA Lin, Li.,Wei, Hulai.,Yi, Juan.,Xie, Bei.,Chen, Jing.,...&Yang, Yue.(2019).Chronic CagA-positive Helicobacter pylori infection with MNNG stimulation synergistically induces mesenchymal and cancer stem cell-like properties in gastric mucosal epithelial cells.JOURNAL OF CELLULAR BIOCHEMISTRY,120(10),17635-17649.
MLA Lin, Li,et al."Chronic CagA-positive Helicobacter pylori infection with MNNG stimulation synergistically induces mesenchymal and cancer stem cell-like properties in gastric mucosal epithelial cells".JOURNAL OF CELLULAR BIOCHEMISTRY 120.10(2019):17635-17649.
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