兰州大学机构库
Long non-coding RNA UCA1 promotes malignant phenotypes of renal cancer cells by modulating the miR-182-5p/DLL4 axis as a ceRNA
Wang, Wei1; Hu, Wentao2; Wang, Ya3; An, Yong1; Song, Lei4; Shang, Panfeng1; Yue, ZJ(岳中瑾)1
2020-01-29
Source PublicationMOLECULAR CANCER   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
EISSN1476-4598
Volume19Issue:1
page numbers17
AbstractBackground Accumulating literatures have indicated that long non-coding RNAs (lncRNAs) are potential biomarkers that play key roles in tumor development and progression. Urothelial cancer associated 1 (UCA1) is a novel lncRNA that acts as a potential biomarker and is involved in the development of cancers. However, the molecular mechanism of UCA1 in renal cancer is still needed to further explore. Methods The relative expression level of UCA1 was determined by Real-Time qPCR in a total of 88 patients with urothelial renal cancer and in different renal cancer cell lines. Loss-of-function experiments were performed to investigate the biological roles of UCA1 and miR-182-5p on renal cancer cell proliferation, migration, apoptosis and tumorigenicity. Comprehensive transcriptional analysis, dual-luciferase reporter assay and western blot etc. were performed to explore the molecular mechanisms underlying the functions of UCA1. Results In this study, we found that UCA1 was significantly up-regulated in renal cancer. Moreover, increased UCA1 expression was positively correlated with differentiation and advanced TNM stage. Further experiments demonstrated that knockdown of UCA1 inhibited malignant phenotypes and Notch signal path of renal cancer cells, and miR-182-5p was reverse function as UCA1. UCA1 functioned as a miRNA sponge to positively regulate the expression of Delta-like ligand 4(DLL4) through sponging miR-182-5p and subsequently promoted malignant phenotypes of renal cancer cells, thus UCA1 playing an oncogenic role and miR-182-5p as an antioncogenic one in renal cancer pathogenesis. Conclusion UCA1-miR-182-5p-DLL4 axis is involved in proliferation and progression of renal cancer. Thus, this study demonstrated that UCA1 plays a critical regulatory role in renal cancer cell and UCA1 may serve as a potential diagnostic biomarker and therapeutic target of renal cancer.
KeywordUCA1 miR-182-5p ceRNA DLL4 Renal cancer
PublisherBMC
DOI10.1186/s12943-020-1132-x
Indexed BySCIE
Language英语
Funding ProjectNational Natural Science Foundations of China[31700720] ; project of Chengguan District Science and Technology Bureau of Lanzhou city[2017SHFZ0029] ; Fundamental Research Funds for the Central Universities[lzujbky-2018-59] ; Lanzhou University Innovation and Entrepreneurship Cultivation Program[cxcy201902] ; Cuiying Graduate Supervisor Applicant Training Program Of Lanzhou University Second Hospital[201806] ; Cuiying Scientific and Technological Innovation Program of Lanzhou University Second Hospital[CY2019-MS17]
WOS Research AreaBiochemistry & Molecular Biology ; Oncology
WOS SubjectBiochemistry & Molecular Biology ; Oncology
WOS IDWOS:000513724300001
PublisherBMC
Original Document TypeArticle
PMID 31996265
ESI Hicited Paper2021-03
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/418247
Collection兰州大学
第二临床医学院
Corresponding AuthorShang, Panfeng; Yue, Zhongjin
Affiliation
1.Lanzhou Univ, Hosp 2, Gansu Nephrourol Clin Ctr, Key Lab Urol Dis Gansu Prov,Dept Urol,Inst Urol, Lanzhou 730030, Gansu, Peoples R China
2.Soochow Univ, Coll Med, Sch Radiat Med & Protect, Collaborat Innovat Ctr Radiol Med,Jiangsu Higher, Suzhou 215123, Peoples R China
3.Lanzhou Univ, Hosp 2, Dept Nephrol, Lanzhou 730000, Gansu, Peoples R China
4.Northwest Min Zu Univ, Sch Med, Lanzhou 730030, Gansu, Peoples R China
First Author AffilicationSecond Clinical School
Corresponding Author AffilicationSecond Clinical School
First Signature AffilicationSecond Clinical School
Recommended Citation
GB/T 7714
Wang, Wei,Hu, Wentao,Wang, Ya,et al. Long non-coding RNA UCA1 promotes malignant phenotypes of renal cancer cells by modulating the miR-182-5p/DLL4 axis as a ceRNA[J]. MOLECULAR CANCER,2020,19(1).
APA Wang, Wei.,Hu, Wentao.,Wang, Ya.,An, Yong.,Song, Lei.,...&Yue, Zhongjin.(2020).Long non-coding RNA UCA1 promotes malignant phenotypes of renal cancer cells by modulating the miR-182-5p/DLL4 axis as a ceRNA.MOLECULAR CANCER,19(1).
MLA Wang, Wei,et al."Long non-coding RNA UCA1 promotes malignant phenotypes of renal cancer cells by modulating the miR-182-5p/DLL4 axis as a ceRNA".MOLECULAR CANCER 19.1(2020).
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