| Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin | |
Omer, Abedelfattah1,2; Al-sharabi, Niyaz2; Qiu, Yingfei1; Xue, Ying2; Li, Y(李屹)1 ; Fujio, Masahito2,3; Mustafa, Kamal2; Xing, Z(邢喆)1,2 | |
| 2020 | |
| Source Publication | Journal of Biomedical Materials Research - Part A Impact Factor & Quartile |
| ISSN | 15524965 |
| EISSN | 1552-4965 |
| Volume | 108Issue:6Pages:1369-1379 |
| page numbers | 11 |
| Abstract | Collagen 1 (COL1) and fibronectin (FN) are extracellular matrix proteins that contribute in cell activity and involve in regulating dental pulp cells (DPCs). The purpose of this study was to investigate the effect of COL1 and FN on the behavior of DPCs. Here, DPCs were grown under three different conditions: COL1 coating, FN coating, and control group without coating. The proliferation and differentiation of DPCs were investigated. DPCs in osteogenic media were able to differentiate into osteoblastic phenotype. The morphological analysis revealed no obvious difference on the shape of cells. Cells had spread well on both coated and noncoated culture plates with slightly more spreading in the coated plates after 24 hr. The MTT analysis did not demonstrate a significant difference at 1 and 3 hr among the groups, but interestingly, the analysis disclosed more cells on the coated plates after longer cultures, which indicated a higher proliferative capacity in response to COL1 and FN. RT-PCR, Western Blotting and mineralization assays did not reveal significant differences between the coated and noncoated surfaces in relation to osteogenic differential potential. Our data suggested that the surface coating of COL1 and FN were able to promote cellular proliferation and the osteogenic differentiation tendency of DPCs was also observed in vitro. © 2020 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. |
| Keyword | biological responses collagen 1 dental pulp cells fibronectin |
| Publisher | WILEY |
| DOI | 10.1002/jbm.a.36908 |
| Indexed By | SCIE ; EI |
| Language | 英语 |
| Funding Project | Fundamental Research Funds for the Central Universities[lzujbkj-2019-ct07] |
| WOS Research Area | Engineering ; Materials Science |
| WOS Subject | Engineering, Biomedical ; Materials Science, Biomaterials |
| WOS ID | WOS:000522795600009 |
| Publisher | John Wiley and Sons Inc., P.O.Box 18667, Newark, NJ 07191-8667, United States |
| EI Accession Number | 20201008275855 |
| EI Keywords | Cell proliferation ; Cells ; Coatings ; Collagen |
| EI Classification Number | Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Coating Materials:813.2 |
| Original Document Type | Journal article (JA) |
| PMID | 32107841 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/418418 |
| Collection | 口腔医学院 |
| Corresponding Author | Li, Yi; Xing, Zhe |
| Affiliation | 1.School of Stomatology, Lanzhou University, Lanzhou, China 2.Centre for Clinical Dental Research, Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway 3.Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan |
| First Author Affilication | School of Stomatology |
| Corresponding Author Affilication | School of Stomatology |
| Recommended Citation GB/T 7714 | Omer, Abedelfattah,Al-sharabi, Niyaz,Qiu, Yingfei,et al. Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin[J]. Journal of Biomedical Materials Research - Part A,2020,108(6):1369-1379. |
| APA | Omer, Abedelfattah.,Al-sharabi, Niyaz.,Qiu, Yingfei.,Xue, Ying.,Li, Yi.,...&Xing, Zhe.(2020).Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin.Journal of Biomedical Materials Research - Part A,108(6),1369-1379. |
| MLA | Omer, Abedelfattah,et al."Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin".Journal of Biomedical Materials Research - Part A 108.6(2020):1369-1379. |
