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Cu(II) nonspecifically binding chromate reductase NfoR promotes Cr(VI) reduction
Han, Huawen1; Zheng, Yuanzhang2; Zhou, Tuoyu1; Liu, Pu1; Li, Xiangkai1
2021-01
Online publication date2020-12
Source PublicationENVIRONMENTAL MICROBIOLOGY   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN1462-2912
EISSN1462-2920
Volume23Issue:1
page numbers16
AbstractCu(II)-enhanced microbial Cr(VI) reduction is common in the environment, yet its mechanism is unknown. The specific activity of chromate reductase, NfoR, from Staphylococcus aureus sp. LZ-01 was augmented 1.5-fold by Cu(II). Isothermal titration calorimetry and spectral data show that Cu(II) binds to NfoR nonspecifically. Further, Cu(II) stimulates the nitrobenzene reduction of NfoR, indicating that Cu(II) promotes electron transfer. The crystal structure of NfoR in complex with CuSO4 (1.46 angstrom) was determined. The overall structure of NfoR-Cu(II) complex is a dimer that covalently binds with FMN and Cu(II)-binding pocket is located at the interface of the NfoR dimer. Structural superposition revealed that NfoR resembles the structure of class II chromate reductase. Site-directed mutagenesis revealed that Leu(46) and Phe(123) were involved in NADH binding, whereas Trp(70) and Ser(45) were the key residues for nitrobenzene binding. Furthermore, His(100) and Asp(171) were preferential affinity sites for Cu(II) and that Cys(163) is an active site for FMN binding. Attenuation reductase activity in C163S can be partially restored to 54% wild type by increasing Cu(II) concentration. Partial restoration indicates dual-channel electron transfer of NfoR via Cu(II) and FMN. We propose a catalytic mechanism for Cu(II)-enhanced NfoR activity in which Cu(I) is formed transiently. Together, the current results provide an insight on Cu (II)-induced enhancement and benefit of Cr(VI) bioremediation.
PublisherWILEY
DOI10.1111/1462-2920.15329
Indexed BySCOPUS ; SCIE
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000595102500001
Original Document TypeArticle
PMID 33201569
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/449206
Collection兰州大学
Corresponding AuthorLi, Xiangkai
Affiliation
1.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou, Peoples R China;
2.Loyola Univ, Dept Chem & Biochem, Chicago, IL 60611 USA
Recommended Citation
GB/T 7714
Han, Huawen,Zheng, Yuanzhang,Zhou, Tuoyu,et al. Cu(II) nonspecifically binding chromate reductase NfoR promotes Cr(VI) reduction[J]. ENVIRONMENTAL MICROBIOLOGY,2021,23(1).
APA Han, Huawen,Zheng, Yuanzhang,Zhou, Tuoyu,Liu, Pu,&Li, Xiangkai.(2021).Cu(II) nonspecifically binding chromate reductase NfoR promotes Cr(VI) reduction.ENVIRONMENTAL MICROBIOLOGY,23(1).
MLA Han, Huawen,et al."Cu(II) nonspecifically binding chromate reductase NfoR promotes Cr(VI) reduction".ENVIRONMENTAL MICROBIOLOGY 23.1(2021).
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