兰州大学机构库
Irradiation-Induced Changes in the Immunogenicity of Lung Cancer Cell Lines: Based on Comparison of X-rays and Carbon Ions
Ran, Juntao1; Wang, Jiangtao2; Dai, Ziying1; Miao, Yandong2; Gan, Jian2; Zhao, Chengpeng3; Guan, Quanlin2,4
2021-04-22
Source PublicationFRONTIERS IN PUBLIC HEALTH   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
EISSN2296-2565
Volume9
page numbers11
AbstractIncreasing the immunogenicity of tumors is considered to be an effective means to improve the synergistic immune effect of radiotherapy. Carbon ions have become ideal radiation for combined immunotherapy due to their particular radiobiological advantages. However, the difference in time and dose of immunogenic changes induced by Carbon ions and X-rays has not yet been fully clarified. To further explore the immunogenicity differences between carbon ions and X-rays induced by radiation in different time windows and dose windows. In this study, we used principal component analysis (PCA) to screen out the marker genes from the single-cell RNA-sequencing (scRNA-seq) of CD8(+) T cells and constructed a protein-protein interaction (PPI) network. Also, ELISA was used to test the exposure levels of HMGB1, IL-10, and TGF-beta under different time windows and dose windows of irradiation with X-rays and carbon ions for A549, H520, and Lewis Lung Carcinoma (LLC) cell lines. The results demonstrated that different marker genes were involved in different processes of immune effect. HMGB1 was significantly enriched in the activated state, while the immunosuppressive factors TGF-beta and IL-10 were mainly enriched in the non-functional state. Both X-rays and Carbon ions promoted the exposure of HMGB1, IL-10, and TGF-beta in a time-dependent manner. X-rays but not Carbon ions increased the HMGB1 exposure level in a dose-dependent manner. Besides, compared with X-rays, carbon ions increased the exposure of HMGB1 while relatively reduced the exposure levels of immunosuppressive factors IL-10 and TGF-beta. Therefore, we speculate that Carbon ions may be more advantageous than conventional X-rays in inducing immune effects.
Keywordirradiationimmunogenicitylung cancerX-rayscarbon ions
PublisherFRONTIERS MEDIA SA
DOI10.3389/fpubh.2021.666282
Indexed BySSCI ; SCOPUS ; SCIE
Language英语
WOS Research AreaPublic, Environmental & Occupational Health
WOS SubjectPublic, Environmental & Occupational Health
WOS IDWOS:000647294300001
Original Document TypeArticle
PMID 33968889
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/450217
Collection兰州大学
第一临床医学院
Corresponding AuthorGuan, Quanlin
Affiliation
1.Lanzhou Univ, Hosp 1, Dept Radiat Oncol, Lanzhou, Peoples R China;
2.Lanzhou Univ, Clin Med Coll 1, Lanzhou, Peoples R China;
3.Lanzhou Univ, Hosp 1, Dept Oncol, Lanzhou, Peoples R China;
4.Lanzhou Univ, Hosp 1, Dept Surg Oncol, Lanzhou, Peoples R China
First Author AffilicationFirst Clinical School
Corresponding Author AffilicationFirst Clinical School
First Signature AffilicationFirst Clinical School
Recommended Citation
GB/T 7714
Ran, Juntao,Wang, Jiangtao,Dai, Ziying,et al. Irradiation-Induced Changes in the Immunogenicity of Lung Cancer Cell Lines: Based on Comparison of X-rays and Carbon Ions[J]. FRONTIERS IN PUBLIC HEALTH,2021,9.
APA Ran, Juntao.,Wang, Jiangtao.,Dai, Ziying.,Miao, Yandong.,Gan, Jian.,...&Guan, Quanlin.(2021).Irradiation-Induced Changes in the Immunogenicity of Lung Cancer Cell Lines: Based on Comparison of X-rays and Carbon Ions.FRONTIERS IN PUBLIC HEALTH,9.
MLA Ran, Juntao,et al."Irradiation-Induced Changes in the Immunogenicity of Lung Cancer Cell Lines: Based on Comparison of X-rays and Carbon Ions".FRONTIERS IN PUBLIC HEALTH 9(2021).
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