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Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model
Xing, Zhe1,2; Jiang, Xiaofeng1; Si, Qingzong1; Finne-Wistrand, Anna3; Liu, Bin1; Xue, Ying2; Mustafa, Kamal2
2021-05
Source PublicationPOLYMERS   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
EISSN2073-4360
Volume13Issue:9
page numbers13
AbstractIt has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.
Keywordbone marrow stromal cellsendothelial cellscopolymer scaffoldendochondral ossificationhost response
PublisherMDPI
DOI10.3390/polym13091521
Indexed ByEI ; SCOPUS ; SCIE
Language英语
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:000650714000001
Original Document TypeArticle
PMID 34065081
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/451009
Collection兰州大学
Corresponding AuthorLiu, Bin; Xue, Ying
Affiliation
1.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China;
2.Univ Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway;
3.KTH Royal Inst Technol, Dept Fibre & Polymer Technol, Teknikringen 56-58, SE-10044 Stockholm, Sweden
Recommended Citation
GB/T 7714
Xing, Zhe,Jiang, Xiaofeng,Si, Qingzong,et al. Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model[J]. POLYMERS,2021,13(9).
APA Xing, Zhe.,Jiang, Xiaofeng.,Si, Qingzong.,Finne-Wistrand, Anna.,Liu, Bin.,...&Mustafa, Kamal.(2021).Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model.POLYMERS,13(9).
MLA Xing, Zhe,et al."Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model".POLYMERS 13.9(2021).
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