| Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy | |
Cheng, J(程菊)1 ; Liu, Di2; Zhao, Lixia3; Zhao, Qianqian4; Zhang, XY(张晓云)4; Wang, B(王蓓)5; Bai, DC(白德成)3 | |
| 2021-07 | |
| Source Publication | NEUROCHEMISTRY INTERNATIONAL
 |
| ISSN | 0197-0186 |
| Volume | 147 |
| Abstract | Cadmium (Cd), a heavy metal with cytotoxicity, can activate autophagy. This study aimed to explore the effects and mechanisms of Potentilla anserine L. polysaccharide (PAP) on autophagy in N2a cells, primary neurons, and the brain of BALB/c mice exposed to Cd. The CCK-8 assay results showed that the cell viability decreased and the number of acidic vesicular organelles, autophagic vacuoles, lysosomes, and dysfunctional mitochondria increased in the cytoplasm of Cd-exposed N2a cells and primary neurons, as revealed by acridine orange staining, monodansylcadaverine staining, and transmission electron microscopy. PAP mitigated Cd-induced neuronal death and characteristic changes in autophagy. The expression of LC3 IILC3 II, Bcl-2, p62, Beclin-1, and PI3K class III was examined by Western blot analysis. Furthermore, the PI3K inhibitor (LY294002 or 3-MA) and/or PAP reversed the Cd-induced upregulated expression of LC3 II, Beclin-1, and PI3K class III, with a synergy between PI3K inhibitor and PAP against Cd-induced autophagy. The findings suggested that PAP partially prevented Cd-induced autophagic cell death in neurons by inhibiting the PI3K class III/Beclin-1 signaling pathway in vitro and in vivo. |
| Keyword | Autophagic cell death BALB c mice Cadmium N2a cells Primary neurons Polysaccharide |
| Publisher | PERGAMON-ELSEVIER SCIENCE LTD |
| DOI | 10.1016/j.neuint.2021.105045 |
| Indexed By | SCOPUS ; SCIE |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| WOS Subject | Biochemistry & Molecular Biology ; Neurosciences |
| WOS ID | WOS:000663457300008 |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/451927 |
| Collection | 基础医学院 药学院 |
| Affiliation | 1.Lanzhou Univ, Inst Genet, Sch Basic Med Sci, Lanzhou 730000, Peoples R China; 2.Gansu Univ Polit Sci & Law, Keylab Evidence Sci Tech Res & Applicat Gansu Pro, Lanzhou 730000, Peoples R China; 3.Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China; 4.Lanzhou Univ, Inst Pharmaceut, Sch Pharm, Lanzhou 730000, Peoples R China; 5.Lanzhou Univ, Sch Basic Med Sci, Lab Ctr Med Sci, Lanzhou 730000, Peoples R China |
| First Author Affilication | School of Basic Medical Sciences |
| Recommended Citation GB/T 7714 | Cheng, Ju,Liu, Di,Zhao, Lixia,et al. Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy[J]. NEUROCHEMISTRY INTERNATIONAL,2021,147. |
| APA | Cheng, Ju.,Liu, Di.,Zhao, Lixia.,Zhao, Qianqian.,Zhang, Xiaoyun.,...&Bai, Decheng.(2021).Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy.NEUROCHEMISTRY INTERNATIONAL,147. |
| MLA | Cheng, Ju,et al."Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy".NEUROCHEMISTRY INTERNATIONAL 147(2021). |
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