兰州大学机构库 >基础医学院
Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy
Cheng, J(程菊)1; Liu, Di2; Zhao, Lixia3; Zhao, Qianqian4; Zhang, XY(张晓云)4; Wang, B(王蓓)5; Bai, DC(白德成)3
2021-07
Source PublicationNEUROCHEMISTRY INTERNATIONAL
ISSN0197-0186
Volume147
AbstractCadmium (Cd), a heavy metal with cytotoxicity, can activate autophagy. This study aimed to explore the effects and mechanisms of Potentilla anserine L. polysaccharide (PAP) on autophagy in N2a cells, primary neurons, and the brain of BALB/c mice exposed to Cd. The CCK-8 assay results showed that the cell viability decreased and the number of acidic vesicular organelles, autophagic vacuoles, lysosomes, and dysfunctional mitochondria increased in the cytoplasm of Cd-exposed N2a cells and primary neurons, as revealed by acridine orange staining, monodansylcadaverine staining, and transmission electron microscopy. PAP mitigated Cd-induced neuronal death and characteristic changes in autophagy. The expression of LC3 IILC3 II, Bcl-2, p62, Beclin-1, and PI3K class III was examined by Western blot analysis. Furthermore, the PI3K inhibitor (LY294002 or 3-MA) and/or PAP reversed the Cd-induced upregulated expression of LC3 II, Beclin-1, and PI3K class III, with a synergy between PI3K inhibitor and PAP against Cd-induced autophagy. The findings suggested that PAP partially prevented Cd-induced autophagic cell death in neurons by inhibiting the PI3K class III/Beclin-1 signaling pathway in vitro and in vivo.
KeywordAutophagic cell death BALB c mice Cadmium N2a cells Primary neurons Polysaccharide
PublisherPERGAMON-ELSEVIER SCIENCE LTD
DOI10.1016/j.neuint.2021.105045
Indexed BySCOPUS ; SCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Neurosciences & Neurology
WOS SubjectBiochemistry & Molecular Biology ; Neurosciences
WOS IDWOS:000663457300008
Original Document TypeArticle
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/451927
Collection基础医学院
药学院
Affiliation1.Lanzhou Univ, Inst Genet, Sch Basic Med Sci, Lanzhou 730000, Peoples R China;
2.Gansu Univ Polit Sci & Law, Keylab Evidence Sci Tech Res & Applicat Gansu Pro, Lanzhou 730000, Peoples R China;
3.Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;
4.Lanzhou Univ, Inst Pharmaceut, Sch Pharm, Lanzhou 730000, Peoples R China;
5.Lanzhou Univ, Sch Basic Med Sci, Lab Ctr Med Sci, Lanzhou 730000, Peoples R China
First Author AffilicationSchool of Basic Medical Sciences
Recommended Citation
GB/T 7714
Cheng, Ju,Liu, Di,Zhao, Lixia,et al. Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy[J]. NEUROCHEMISTRY INTERNATIONAL,2021,147.
APA Cheng, Ju.,Liu, Di.,Zhao, Lixia.,Zhao, Qianqian.,Zhang, Xiaoyun.,...&Bai, Decheng.(2021).Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy.NEUROCHEMISTRY INTERNATIONAL,147.
MLA Cheng, Ju,et al."Potentilla anserine L. polysaccharide inhibits cadmium-induced neurotoxicity by attenuating autophagy".NEUROCHEMISTRY INTERNATIONAL 147(2021).
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