An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants

doi:10.1080/13102818.2021.1986132

兰州大学机构库

	An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants
	Wang, Jun-Li; Liang, Luo-Yu; Lang, Nan; Li, Yu-Yang; Guo, Wang; Cui, Jian; Zou, Ya-Jie; Liu, Hai-Qin; Fei, Qiong-Hui; Li, Xiao-Feng; Guo, Guang-Qin; Wu, Lei
	2021
Source Publication	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT Impact Factor & Quartile
ISSN	1310-2818
EISSN	1314-3530
Volume	35 Issue:1 Pages:1850-1857
page numbers	8
Abstract	CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 mediated gene editing systems have been used in a variety of organisms, including plants. This system can efficiently edit target genes to generate mutants, especially multiplex mutants, which are invaluable genetic materials for gene functional studies. Although the use of traditional hybridization technology to construct multiplex mutants has been widely used in plant research, the complex screening and identification, long construction cycle and other problems have always troubled researchers. Therefore, in the current plant science research, the use of CRISPR-Cas9 mediated multiplex genome editing to construct multiplex mutants has become a mainstream technology. Here, we designed an improved plant CRISPR-Cas9 mediated multiplex genome vector system. The In-fusion cloning technology was selected to conveniently assemble multiple tandem sgRNA (Single-guide RNA) expression frames. In this system, Cas9-free plants can be easily selected by examining mCherry fluorescence in T-2 transgenic plants. By using this system, Cas9-free homozygous triple mutant was obtained, demonstrating the availability of our system.
Keyword	CRISPR-Cas9 multiplex genome editing In-Fusion cloning technology Cas9-free
Publisher	TAYLOR & FRANCIS LTD
DOI	10.1080/13102818.2021.1986132
Indexed By	SCIE
Language	英语
WOS Research Area	Biotechnology & Applied Microbiology
WOS Subject	Biotechnology & Applied Microbiology
WOS ID	WOS:000744290200001
Original Document Type	Article
Citation statistics
Document Type	期刊论文
Identifier	https://ir.lzu.edu.cn/handle/262010/479793
Collection	兰州大学
Corresponding Author	Wu, Lei
Affiliation	Lanzhou Univ, Sch Life Sci, Dept MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China
Recommended Citation GB/T 7714	Wang, Jun-Li,Liang, Luo-Yu,Lang, Nan,et al. An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2021,35(1):1850-1857.
APA	Wang, Jun-Li.,Liang, Luo-Yu.,Lang, Nan.,Li, Yu-Yang.,Guo, Wang.,...&Wu, Lei.(2021).An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,35(1),1850-1857.
MLA	Wang, Jun-Li,et al."An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 35.1(2021):1850-1857.

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