| IL-7 promoted the development of thymic DN3 cells in aged mice via DNA demethylation of Bcl2 and c-Myc genes | |
| Han, Jiangyuan1,2,6; Ma, Yanlin1,2,6; Lv, Wei1,2,6; Wang, Juan1,2,6; Wu, Yu4; Niu, Hongxia1,2,6; Mi, Youjun1,3,6; Li, Fei1,2,6; Zhu, Bingdong1,2,5,6 | |
| 2022-07-01 | |
| Online publication date | 2022-04 |
| Source Publication | MOLECULAR IMMUNOLOGY Impact Factor & Quartile |
| ISSN | 0161-5890 |
| EISSN | 1872-9142 |
| Volume | 147Pages:21-29 |
| page numbers | 9 |
| Abstract | IL-7 promotes the development of thymic double negative (DN) T cells during beta-selection, which might contribute to the remission of aging-associated thymic involution. Methylation levels of CpG sites is correlated with aging and modulates the development. To determine the involvement of DNA methylation/demethylation instructed by IL-7 signaling during the expansion of double negative (DN) T cells, the aged mice were treated with recombinant Adeno-Associated Virus 2-mediated IL-7 (rAAV2-IL-7) and the DNA methylation/demethylation modifications in this process were analyzed. The results showed that rAAV2-IL-7 increased the number of thymocytes, especially the DN3 thymocytes during beta-selection in aged mice. With aging, the methylation levels of Bcl2 and c-Myc promoter regions were increased in DN3 cells. Following rAAV2-IL-7 treatment, DNA methyl-transferase Dnmt3a and Dnmt3b decreased, DNA demethylation factors TET2 and TET3 increased, and the methylation levels of Bcl2 and c-Myc in DN3 cells were reduced during DN3 stage in aged mice, consequently, resulting in the upregulation of Bcl2 and c-Myc and the larger increase of DN3 cells in thymus. In conclusion, these findings showed that Bcl2 and c-Myc genes of DN3 cells had an increased DNA methylation levels in aged mice compared to the young, and the hypermethylation in aged mice could be restored following rAAV2-IL-7 treatment. |
| Keyword | IL-7 Ageing DNA demethylation DN3 cells Bcl2 c-Myc |
| Publisher | PERGAMON-ELSEVIER SCIENCE LTD |
| DOI | 10.1016/j.molimm.2022.04.013 |
| Indexed By | SCIE |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Immunology |
| WOS Subject | Biochemistry & Molecular Biology ; Immunology |
| WOS ID | WOS:000797833900003 |
| Original Document Type | Article |
| PMID | 3550051 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/479907 |
| Collection | 兰州大学 |
| Corresponding Author | Zhu, Bingdong |
| Affiliation | 1.Lanzhou Univ, Sch Basic Med Sci, Gansu Prov Key Lab Evidence Based Med & Clin Trans, Lanzhou 730000, Peoples R China; 2.Lanzhou Univ, Inst Pathogen Biol, Sch Basic Med Sci, Lanzhou 730000, Peoples R China; 3.Lanzhou Univ, Inst Pathophysiol, Sch Basic Med Sci, Lanzhou 730000, Peoples R China; 4.Gansu Prov Hosp, Inst Clin Res & Translat Med, Lanzhou 730000, Peoples R China; 5.Lanzhou Univ, Inst Pathogen Biol, Sch Basic Med Sci, 199 West Donggang Rd, Lanzhou 730000, Peoples R China; 6.Lanzhou Univ, Lanzhou Ctr TB Res, Sch Basic Med Sci, Lanzhou 730000, Peoples R China |
| Recommended Citation GB/T 7714 | Han, Jiangyuan,Ma, Yanlin,Lv, Wei,et al. IL-7 promoted the development of thymic DN3 cells in aged mice via DNA demethylation of Bcl2 and c-Myc genes[J]. MOLECULAR IMMUNOLOGY,2022,147:21-29. |
| APA | Han, Jiangyuan.,Ma, Yanlin.,Lv, Wei.,Wang, Juan.,Wu, Yu.,...&Zhu, Bingdong.(2022).IL-7 promoted the development of thymic DN3 cells in aged mice via DNA demethylation of Bcl2 and c-Myc genes.MOLECULAR IMMUNOLOGY,147,21-29. |
| MLA | Han, Jiangyuan,et al."IL-7 promoted the development of thymic DN3 cells in aged mice via DNA demethylation of Bcl2 and c-Myc genes".MOLECULAR IMMUNOLOGY 147(2022):21-29. |
| Files in This Item: | There are no files associated with this item. | ||||
