兰州大学机构库 >基础医学院
Protective effect of resveratrol on rat cardiomyocyte H9C2 cells injured by hypoxia/reoxygenation by regulating mitochondrial autophagy via PTEN-induced putative kinase protein 1/Parkinson disease protein 2 signaling pathway
Zhao Lixia1,2,3; Sun Wei4; Bai, DC(白德成)1
2022-04-01
Source PublicationJOURNAL OF TRADITIONAL CHINESE MEDICINE
ISSN0255-2922
Volume42Issue:2Pages:176-186
AbstractOBJECTIVE: To investigate the protective effect of resveratrol on cardiomyocytes after hypoxia/reoxygenation intervention based on PTEN-induced putative kinase protein 1/Parkinson disease protein 2 (PINK1/PARKIN) signaling pathway. METHODS: 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to detect the effect of resveratrol on the viability of H9C2 cells; the hypoxia/reoxygenation (H/R) model was established in tri-gas incubator; 2', 7'-Dichlorofluorescin diacetate staining was used to measure the content of reactive oxygen species (ROS); the changes of mitochondrial membrane potential was determined by 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide staining; the changes of mitochondrial respiratory chain complex activity was evaluated by enzyme activity kits; flow cytometry was used to detect the ratio of apoptotic cells; transmission electron microscope was used to observe the ultrastructure of H9C2 cells; Western blot was used to detect the protein changes of mitochondrial 20 kDa outer membrane protein (TOM20), translocase of inner mitochondrial membrane 23 (TIM23), presenilins associated rhomboid-like protein (PARL), PINK1, PARKIN and mitofusin 1 (Mfn1), mitofusin 2 (Mfn2), phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa (P62), microtubule-associated protein 1 light chain 3 beta (LC3B); the mRNA levels of PINK1 and PARKIN was detected by quantitative polymerase chain reaction; immunoprecipitation assay was used to detect the interaction between PARKIN and Ubiquitin. RESULTS: Resveratrol could inhibit the proliferation of H9C2 cells in a time- and concentration- dependent manner; however, pretreatment with low cytotoxic resveratrol could reduce the H/R-induced increase in cellular ROS levels, alleviate the loss of mitochondrial membrane potential induced by H/R, inhibit H/R-induced apoptosis of H9C2 cells, and protect the mitochondrial structure and respiratory chain of H9C2 cells from H/R damage. Resveratrol could further increase the levels of p62, PINK1, PARKIN protein, the expression of PINK1, PARKIN mRNA and the ratio of LC3BII/LC3B I in H/R-induced H9C2 cells, inhibit the interaction between PARKIN and Ubiquitin in H/R-induced H9C2 cells, and further reduce the expression of TOM20, TIM23, PARL, Mfn1 and Mfn2 protein in H/R-induced H9C2 cells. The effect of resveratrol is consistent with that of autophagy activator on H/R-induced H9C2 cells. CONCLUSIONS: Resveratrol can protect H9C2 cells from H/R injury, which may be related to resveratrol promoting mitochondrial autophagy by activating PINK1/PARKIN signaling pathway. (C) 2022 JTCM. All rights reserved.
Keywordresveratrol myocytes cardiac hypoxia PTEN phosphohydrolase Parkinson disease associated proteins mitochondrial autophagy
PublisherJOURNAL TRADITIONAL CHINESE MED
DOI10.19852/j.cnki.jtcm.20220311.002
Indexed BySCIE
Language英语
WOS Research AreaIntegrative & Complementary Medicine
WOS SubjectIntegrative & Complementary Medicine
WOS IDWOS:000792400200001
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/480014
Collection基础医学院
Affiliation1.Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;
2.Gansu Univ Chinese Med, Sch Nursing, Lanzhou 730000, Peoples R China;
3.Gansu Univ Chinese Med, Key Lab Dunhuang Med, Minist Educ, Lanzhou 730000, Peoples R China;
4.Lanzhou Univ, Hosp 1, Cardiac Surg, Lanzhou 730000, Gansu, Peoples R China
First Author AffilicationSchool of Basic Medical Sciences
Recommended Citation
GB/T 7714
Zhao Lixia,Sun Wei,Bai Decheng. Protective effect of resveratrol on rat cardiomyocyte H9C2 cells injured by hypoxia/reoxygenation by regulating mitochondrial autophagy via PTEN-induced putative kinase protein 1/Parkinson disease protein 2 signaling pathway[J]. JOURNAL OF TRADITIONAL CHINESE MEDICINE,2022,42(2):176-186.
APA Zhao Lixia,Sun Wei,&Bai Decheng.(2022).Protective effect of resveratrol on rat cardiomyocyte H9C2 cells injured by hypoxia/reoxygenation by regulating mitochondrial autophagy via PTEN-induced putative kinase protein 1/Parkinson disease protein 2 signaling pathway.JOURNAL OF TRADITIONAL CHINESE MEDICINE,42(2),176-186.
MLA Zhao Lixia,et al."Protective effect of resveratrol on rat cardiomyocyte H9C2 cells injured by hypoxia/reoxygenation by regulating mitochondrial autophagy via PTEN-induced putative kinase protein 1/Parkinson disease protein 2 signaling pathway".JOURNAL OF TRADITIONAL CHINESE MEDICINE 42.2(2022):176-186.
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