| Antifungal activity and preliminary mechanism of pristimerin against Sclerotinia sclerotiorum | |
Zhao, Wen-Bin1; Zhao, Zhong-Min1; Ma, Yue1; Li, An-Ping3; Zhang, ZJ(张智军)1 ; Hu, Yong-Mei1; Zhou, Yong1; Wang, Rui4; Luo, Xiong-Fei1; Zhang, Bao-Qi1; Wang, Yu-Ling2; Hu, Guan-Fang2; Liu, YQ(刘映前)1 | |
| 2022-10-01 | |
| Source Publication | INDUSTRIAL CROPS AND PRODUCTS Impact Factor & Quartile |
| ISSN | 0926-6690 |
| Volume | 185 |
| Abstract | Sclerotinia sclerotiorum (Lib.) de Bary is a crop-threatening fungus. Natural products are ideal antimicrobial agents to combat pathogens. They are considered a promising alternative to traditional synthetic fungicides due to low residual toxicity and possibly different modes of action on organisms. This work aims to develop a leading compound from natural products. Here, the antifungal activity of ten pentacyclic triterpenoids, including celastrol, pristimerin, asiatic acid, maslinic acid, ursolic acid, oleanic acid, quillaic acid, glycyrrhetinic acid, madecassic acid and corosolic acid were evaluated. Pristimerin exhibited an excellent activity against Sclerotinia sclerotiorum (S. sclerotiorum) with an EC50 value of 1.36 µg/mL. Subsequently, the mode of action of pristimerin on S. sclerotiorum was further characterized by RNA-Seq-Based transcriptome profile. Pristimerin mainly inhibited oxidative phosphorylation, citrate cycle (TCA cycle) and blocked energy metabolism, and it also promoted the accumulation of ROS and disrupted the balance of the active oxygen metabolism system by affecting the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) in the mycelial cells, and ultimately led to cell death. And beyond that, mitochondrial damage and changes in mycelial cells were also observed by transmission electron microscope (TEM) and scanning electron microscope (SEM). In general, these findings showed a molecular level perspective on the antifungal mechanism of pristimerin against S. sclerotiorum, and it could be used as a potential fungicide against S. sclerotiorum. © 2022 |
| Keyword | Antifungal agents Cell death Enzymes Fungi Fungicides High resolution transmission electron microscopy Metabolism Mitochondria Scanning electron microscopy Antifungal activities Celastrols Mitochondrial function Mode of action Mycelial cells Natural products Pentacyclic triterpenoids Pristimerin RNA-seq Sclerotinia Sclerotiorum S. sclerotiorum RNA-Seq |
| Publisher | Elsevier B.V. |
| DOI | 10.1016/j.indcrop.2022.115124 |
| Indexed By | EI ; SCIE |
| Language | 英语 |
| WOS Research Area | Agriculture |
| WOS Subject | Agricultural Engineering ; Agronomy |
| WOS ID | WOS:000808530800004 |
| EI Accession Number | 20222412213072 |
| EI Keywords | RNA |
| EI Classification Number | 461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.9 Biology ; 741.3 Optical Devices and Systems ; 804.1 Organic Compounds |
| Original Document Type | Journal article (JA) ; Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/481973 |
| Collection | 药学院 |
| Corresponding Author | Liu, Ying-Qian |
| Affiliation | 1.School of Pharmacy, Lanzhou University, Lanzhou; 730000, China; 2.Gansu Academy of Agricultural Sciences, Lanzhou; 730000, China; 3.Gansu Institute for Drug Control, Lanzhou; 730000, China; 4.Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang; 261061, China |
| First Author Affilication | School of Pharmacy |
| Corresponding Author Affilication | School of Pharmacy |
| Recommended Citation GB/T 7714 | Zhao, Wen-Bin,Zhao, Zhong-Min,Ma, Yue,et al. Antifungal activity and preliminary mechanism of pristimerin against Sclerotinia sclerotiorum[J]. INDUSTRIAL CROPS AND PRODUCTS,2022,185. |
| APA | Zhao, Wen-Bin.,Zhao, Zhong-Min.,Ma, Yue.,Li, An-Ping.,Zhang, Zhi-Jun.,...&Liu, Ying-Qian.(2022).Antifungal activity and preliminary mechanism of pristimerin against Sclerotinia sclerotiorum.INDUSTRIAL CROPS AND PRODUCTS,185. |
| MLA | Zhao, Wen-Bin,et al."Antifungal activity and preliminary mechanism of pristimerin against Sclerotinia sclerotiorum".INDUSTRIAL CROPS AND PRODUCTS 185(2022). |
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