|A fully functional palmtop microchip electrophoresis analyzer with laser-induced fluorescence detection|
|Wang, Yuanhang; Wu, Jing; Sun, Ping; Yu, Jie; Pu, Qiaosheng|
|Online publication date||2022-09
|Source Publication||SENSORS AND ACTUATORS B-CHEMICAL
Impact Factor & Quartile|
|Abstract||Microchip electrophoresis (MCE) is an ideal tool for rapid on-site analysis of multiple components, therefore the demand for portable MCE devices is increasing. In this work, a fully functional palmtop MCE analyzer was built, integrating units of sample injection, laser-induced fluorescence (LIF) detection, high-voltage (HV), imaging and data acquisition. Frames were fabricated with 3D printing to encapsulate miniaturized components selected with balanced consideration on size, cost and performance. The LIF unit contains a laser diode as the excitation light source, a collimating lens used in the commercial laser diode as the focusing lens and an avalanche photodiode (APD) for the laser distance meter as the photoelectric converter. The sample injection was achieved by negative pressure induced pinching. The total cost of the device is approximately $600 with a size of 89 × 98 × 98 mm3 and a weight of 350 g. It can be powered by a laptop or a mobile power bank. The limit of detection (LOD) measured under a typical MCE condition for sodium fluorescein could be 110 pM. The applicability of the device was demonstrated with the detection of rhodamines and DNA fragments. © 2022 Elsevier B.V.|
Laser produced plasmas
Laser induced fluorescence
Laser induced fluorescence detection
|WOS Research Area||Chemistry
; Instruments & Instrumentation
|WOS Subject||Chemistry, Analytical
; Instruments & Instrumentation
|EI Accession Number||20223812767483
|EI Keywords||3D printers
|EI Classification Number||701.1 Electricity: Basic Concepts and Phenomena
; 723.2 Data Processing and Image Processing
; 741.1 Light/Optics
; 744.1 Lasers, General
; 744.4.1 Semiconductor Lasers
; 744.9 Laser Applications
; 745.1.1 Printing Equipment
; 801.3 Colloid Chemistry
; 801.4 Physical Chemistry
; 801.4.1 Electrochemistry
; 911 Cost and Value Engineering
; Industrial Economics
; 932.3 Plasma Physics
|Original Document Type||Journal article (JA)
|Corresponding Author||Pu, Qiaosheng|
National & Local Joint Engineering Laboratory for Optical Conversion Materials and Technology, School of Materials and Energy, Lanzhou University, Lanzhou; 730000, China
|First Author Affilication||Lanzhou University
|Corresponding Author Affilication||Lanzhou University
Wang, Yuanhang,Wu, Jing,Sun, Ping,et al. A fully functional palmtop microchip electrophoresis analyzer with laser-induced fluorescence detection[J].
SENSORS AND ACTUATORS B-CHEMICAL,2022,372.
Wang, Yuanhang,Wu, Jing,Sun, Ping,Yu, Jie,&Pu, Qiaosheng.(2022).A fully functional palmtop microchip electrophoresis analyzer with laser-induced fluorescence detection.SENSORS AND ACTUATORS B-CHEMICAL,372.
Wang, Yuanhang,et al."A fully functional palmtop microchip electrophoresis analyzer with laser-induced fluorescence detection".SENSORS AND ACTUATORS B-CHEMICAL 372(2022).
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