| Discovery of cryptolepine derivatives as novel promising agents against phytopathogenic bacteria | |
He, Ying-Hui1,2; Chu, Qing-Ru2; Zhang, Shao-Yong1; Guo, Li-Rong2; Ma, Yue2; Zhang, Bao-Qi2; Zhang, ZJ(张智军)2 ; Zhao, Wen-Bin2; Hu, Yong-Mei2; Yang, Chen-Jie2; Du, Sha-Sha2; Wu, Tian-Lin2; Liu, YQ(刘映前)1,2,3 | |
| 2022 | |
| Online publication date | 2022-10 |
| Source Publication | Frontiers of Chemical Science and Engineering Impact Factor & Quartile |
| ISSN | 2095-0179 |
| EISSN | 2095-0187 |
| Volume | 17Issue:2Pages:156-166 |
| page numbers | 11 |
| Abstract | To ensure the production of food crops, a series of cryptolepine derivatives were synthesised, after which their antibacterial activities and mechanism of action against three plant pathogens were investigated. Our bioassay results indicated that most of the target compounds displayed potent inhibitory effects against Xanthomonas oryzae (X. oryzae) and Xanthomonas axonopodis pv. citri (X. axonopodis pv. c.). Remarkably, compound 9 exhibited the best in vitro antibacterial activity against X. oryzae, with a minimum inhibitory concentration (MIC) value of 0.78 μg·mL−1. Compound 2 exhibited the best in vitro antibacterial activity against X. axonopodis pv. c., with an MIC value of 0.39 μg·mL−1. These activities were superior to those of copper quinolate (MIC = 6.25, 25 μg·mL−1) and thiodiazole copper (MIC = 100, 200 μg·mL−1) against X. oryzae and X. axonopodis pv. c. In vivo experiments demonstrated the promising applicability of compound 9 for the control of rice bacterial infections. Furthermore, compound 9 was selected as a candidate to conduct preliminary analyses of the antibacterial mechanisms of cryptolepine derivatives. Scanning electron microscopy and transmission electron microscopy observations, extracellular polysaccharide production, biofilm formation, transcriptomic, quantitative reverse transcription-polymerase chain reaction analyses, and molecular docking assays were performed. Collectively, our findings demonstrated that compound 9 might act via multifarious mechanisms to down-regulate virulence factors and cause cell death. [Figure not available: see fulltext.] © 2022, Higher Education Press. |
| Keyword | Bacteria Cell death High resolution transmission electron microscopy Microwave integrated circuits Polymerase chain reaction Scanning electron microscopy Anti bacterial activity Antibacterial mechanisms Concentration values Cryptolepine derivative Food crops In vitro Mechanism of action Minimum inhibitory concentration Phytopathogenic bacteria Xanthomonas oryzae |
| Publisher | Higher Education Press Limited Company |
| DOI | 10.1007/s11705-022-2196-5 |
| Indexed By | EI ; SCIE |
| Language | 英语 |
| WOS Research Area | Engineering |
| WOS Subject | Engineering, Chemical |
| WOS ID | WOS:000864551300001 |
| EI Accession Number | 20224112882440 |
| EI Keywords | Copper |
| EI Classification Number | 461.9 Biology ; 544.1 Copper ; 714.2 Semiconductor Devices and Integrated Circuits ; 741.3 Optical Devices and Systems ; 801.2 Biochemistry |
| Original Document Type | Article in Press |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.lzu.edu.cn/handle/262010/492361 |
| Collection | 药学院 |
| Corresponding Author | Liu, Ying-Qian |
| Affiliation | 1.Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science, Huzhou University, Huzhou; 313000, China; 2.School of Pharmacy, Lanzhou University, Lanzhou; 730000, China; 3.Laboratory of Grassland Agro-ecosystems, Lanzhou University, Lanzhou; 730000, China |
| First Author Affilication | School of Pharmacy |
| Corresponding Author Affilication | School of Pharmacy; Lanzhou University |
| Recommended Citation GB/T 7714 | He, Ying-Hui,Chu, Qing-Ru,Zhang, Shao-Yong,et al. Discovery of cryptolepine derivatives as novel promising agents against phytopathogenic bacteria[J]. Frontiers of Chemical Science and Engineering,2022,17(2):156-166. |
| APA | He, Ying-Hui.,Chu, Qing-Ru.,Zhang, Shao-Yong.,Guo, Li-Rong.,Ma, Yue.,...&Liu, Ying-Qian.(2022).Discovery of cryptolepine derivatives as novel promising agents against phytopathogenic bacteria.Frontiers of Chemical Science and Engineering,17(2),156-166. |
| MLA | He, Ying-Hui,et al."Discovery of cryptolepine derivatives as novel promising agents against phytopathogenic bacteria".Frontiers of Chemical Science and Engineering 17.2(2022):156-166. |
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