兰州大学机构库
Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway
Wang, Jia1; Li, Wanxin1; He, Xuxia1; Li, Simei1; Pan, Hongwei1; Yin, LH(殷丽华)1,2
2023-12-31
Source PublicationPLATELETS   Impact Factor & Quartile
ISSN0953-7104
Volume34Issue:1
page numbers10
AbstractBone regeneration in dentistry is a dynamic approach for treating critical size bone defects that are unlikely to self-heal. Human bone marrow stem cell (hBMSCs) therapies are being tested clinically for various disorders and have remarkable clinical advancements in bone regeneration. Injectable platelet-rich fibrin (i-PRF), which is obtained from autologous blood centrifuged at 700 rpm (60 G) for 3 min can promote osteogenic differentiation of this cell, but the mechanism remains unclear. The objectives of this study were to explore the contents of i-PRF further and investigate its effect on the cell behavior of hBMSCs and the underlying molecular mechanisms. The results showed that i-PRF contained 41 cytokines, including macrophage colony-stimulating factor (M-CSF) and beta-nerve growth factor (beta-NGF), which had not been reported before. The Cell Counting Kit-8 and wound healing assay showed that 10% and 20% i-PRF improved the proliferation rate and the migration capacity of hBMSCs without toxicity to cells. Besides, the expression of osteogenic markers and the capacity to form mineralized nodules of hBMSCs were promoted by 20% i-PRF. Furthermore, i-PRF activated the ERK pathway, and the ERK inhibitor attenuated its effects. In summary, i-PRF promotes hBMSCs proliferation and migration and facilitates cell osteogenesis through the ERK pathway, which has promising potential in bone regeneration. Plain Language Summary What is the context? Bone defects caused by trauma or tumor is a great challenge in clinical practice. However, there is the good news that the bone defect in the oral can self-regenerate, the bone remodeling may take several months to several years and shows apparent individual differences. Different strategies, surgical techniques, and materials have been employed to induce an optimal outcome in guided bone regeneration. Blood products have been widely used in dentistry due to their excellent biocompatibility, growth factor content, ease of collection, and ability to be produced by the human body. Limited data suggest that Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells, but further evidence is needed to quantify this effect. What is new? It is unclear how many growth factors i-PRF contains in previous studies, so we detected 41 kinds of growth factors, more than has been previously appreciated, and found that all growth factors were measured in the samples, and the difference was in the amount of expression. In our research, we explored the role of i-PRF in the osteogenesis of hBMSCs through the effects of different concentrations of i-PRF on the proliferation, migration, and osteogenic differentiation of hBMSCs. Currently, most current research focuses on observing phenomena, and we wondered by what mechanism the i-PRF regulates stem cell function. We found that i-PRF can regulate the molecular mechanism of the osteogenic differentiation of hBMSCs in vitro by activating the MAPK/ERK signaling pathway. What is the impact? I-PRF promotes hBMSCs proliferation and migration and facilitates cell osteogenesis through the ERK pathway. The favorable cytobiological effects of i-PRF on hBMSCs might be the basis for i-PRF applications in bone regenerative.
KeywordERK1 2 hBMSCs injectable platelet-rich fibrin osteogenic differentiation
PublisherTAYLOR & FRANCIS INC
DOI10.1080/09537104.2022.2159020
Indexed BySCIE
Language英语
WOS Research AreaCell Biology ; Hematology
WOS SubjectCell Biology ; Hematology
WOS IDWOS:000914207700001
Original Document TypeArticle
PMID 36644947
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/499749
Collection兰州大学
口腔医学院
Corresponding AuthorYin, Lihua
Affiliation
1.Lanzhou Univ, Dept Implantol, Sch Hosp Stomatol, Lanzhou, Peoples R China;
2.Lanzhou Univ, Sch Hosp Stomatol, Dept Implantol, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China
First Author AffilicationSchool of Stomatology
Corresponding Author AffilicationSchool of Stomatology
Recommended Citation
GB/T 7714
Wang, Jia,Li, Wanxin,He, Xuxia,et al. Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway[J]. PLATELETS,2023,34(1).
APA Wang, Jia,Li, Wanxin,He, Xuxia,Li, Simei,Pan, Hongwei,&Yin, Lihua.(2023).Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway.PLATELETS,34(1).
MLA Wang, Jia,et al."Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway".PLATELETS 34.1(2023).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Wang, Jia]'s Articles
[Li, Wanxin]'s Articles
[He, Xuxia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jia]'s Articles
[Li, Wanxin]'s Articles
[He, Xuxia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jia]'s Articles
[Li, Wanxin]'s Articles
[He, Xuxia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.